2aas

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HIGH-RESOLUTION THREE-DIMENSIONAL STRUCTURE OF RIBONUCLEASE A IN SOLUTION BY NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

Overview

High-resolution three-dimensional structures of bovine pancreatic, ribonuclease A in aqueous solution have been determined by nuclear, magnetic resonance (NMR) spectroscopy combined with restrained molecular, dynamics calculations. The structures are based on: (1) 464 interproton, distance constraints with accurate upper and lower limits, determined from, build-up rates of nuclear Overhauser effects (NOE) by using the complete, relaxation matrix; (2) 999 more approximate upper limits for interproton, distances; and (3) 42 dihedral angle constraints (37 for phi and 5 for chi, 1). A total of 16 structures were calculated, which show a, root-mean-square (r.m.s.) deviation of 0.66 A for the backbone atoms and, 1.68 A for all heavy-atoms. The converged structures are highly similar to, those found in the crystal state. r.m.s. deviation of backbone atom, positions in the crystal as compared to those in the average solution, structure is 0.92 A. Observed differences are concentrated in loop regions, and in the neighborhood of His119 and His48 side-chains. Dynamic aspects, such as H/D amide proton exchange and side-chain mobility have been, examined.

About this Structure

2AAS is a Single protein structure of sequence from Bos taurus. Active as Pancreatic ribonuclease, with EC number 3.1.27.5 Full crystallographic information is available from OCA.

Reference

High-resolution three-dimensional structure of ribonuclease A in solution by nuclear magnetic resonance spectroscopy., Santoro J, Gonzalez C, Bruix M, Neira JL, Nieto JL, Herranz J, Rico M, J Mol Biol. 1993 Feb 5;229(3):722-34. PMID:8381876

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