2agk

From Proteopedia

Revision as of 05:59, 21 November 2007

Structure of S. cerevisiae His6 protein

Overview

The Saccharomyces cerevisiae His6 gene codes for the enzyme, phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxamide isomerase, catalyzing the fourth step in histidine biosynthesis. To get an insight, into the structure and function of this enzyme, we determined its X-ray, structure at a resolution of 1.30 A using the anomalous diffraction signal, of the protein's sulphur atoms at 1.77 A wavelength. His6 folds in an, (alpha/beta)8 barrel similar to HisA, which performs the same function in, bacteria and archaea. We found a citrate molecule from the buffer bound in, a pocket near the expected position of the active site and used it to, model the open form of the substrate (phosphoribulosyl moiety), which is a, reaction intermediate. This model enables us to identify catalytic, residues and to propose a reaction mechanism where two aspartates act as, acid/base catalysts: Asp134 as a proton donor for ring opening, and Asp9, as a proton acceptor and donor during enolization of the aminoaldose. Asp9, is conserved in yeast His6 and bacterial or archaeal HisA sequences, and, Asp134 has equivalents in both HisA and TrpF, but they occur at a, different position in the protein sequence.

About this Structure

2AGK is a Single protein structure of sequence from Saccharomyces cerevisiae with CL, CIT and EDO as ligands. Active as 1-(5-phosphoribosyl)-5- ((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase, with EC number 5.3.1.16 Full crystallographic information is available from OCA.

Reference

Crystal structure of the yeast His6 enzyme suggests a reaction mechanism., Quevillon-Cheruel S, Leulliot N, Graille M, Blondeau K, Janin J, van Tilbeurgh H, Protein Sci. 2006 Jun;15(6):1516-21. PMID:16731983

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