2ai1

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(New page: 200px<br /><applet load="2ai1" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ai1, resolution 2.00&Aring;" /> '''Purine nucleoside ph...)
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Revision as of 06:00, 21 November 2007


2ai1, resolution 2.00Å

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Purine nucleoside phosphorylase from calf spleen

Overview

In an effort to develop potent multisubstrate-analog inhibitors of purine, nucleoside phosphorylase (PNP), three nucleoside phosphonates were, designed utilizing structural information from the previously reported, structures of complexes of bovine PNP with substrates and products. The, nucleoside phosphonates contain an acetal linkage at the O2' and O3', positions and a two-C-atom spacer between the ribose and phosphate, moieties. The linkage enables the compounds to simultaneously occupy the, purine-, ribose- and phosphate-binding sites. The chemical syntheses, inhibition profiles and structural characterization of these novel, multisubstrate analog inhibitors with bovine PNP are described.

About this Structure

2AI1 is a Single protein structure of sequence from Bos taurus with MG, ZN, 144 and P1G as ligands. Active as Purine-nucleoside phosphorylase, with EC number 2.4.2.1 Full crystallographic information is available from OCA.

Reference

Novel multisubstrate inhibitors of mammalian purine nucleoside phosphorylase., Toms AV, Wang W, Li Y, Ganem B, Ealick SE, Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1449-58. Epub 2005, Oct 19. PMID:16239721

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