1a39
From Proteopedia
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(New page: 200px<br /> <applet load="1a39" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a39, resolution 2.2Å" /> '''HUMICOLA INSOLENS EN...)
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Revision as of 19:39, 29 October 2007
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HUMICOLA INSOLENS ENDOCELLULASE EGI S37W, P39W DOUBLE-MUTANT
Overview
Family 7 of the glycosyl hydrolases contains both endoglucanases and, cellobiohydrolases. In addition to their different catalytic activities on, crystalline substrates, the cellobiohydrolases differ from the, endoglucanases in their activity on longer soluble substrates, indicative, of a greater number of subsites on the enzyme. A double mutant (S37W, P39W) of the Humicola insolens endoglucanase I (EG I) has been constructed, in order to mimic aspects of the subsite structure of the corresponding, family 7 cellobiohydrolase, cellobiohydrolase-I (CBH I). The 3-D crystal, structure of the double mutant has been solved and refined to a, crystallographic R-factor of 0.17 at a resolution of 2.2 A (1 A = 0.1 nm)., The two mutant tryptophans are clearly visible in the electron density and, are ... [(full description)]
About this Structure
1A39 is a [Single protein] structure of sequence from [Humicola insolens] with NAG as [ligand]. Active as [[1]], with EC number [3.2.1.4]. Full crystallographic information is available from [OCA].
Reference
Oligosaccharide specificity of a family 7 endoglucanase: insertion of potential sugar-binding subsites., Davies GJ, Ducros V, Lewis RJ, Borchert TV, Schulein M, J Biotechnol. 1997 Sep 16;57(1-3):91-100. PMID:9335168
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