2ab6
From Proteopedia
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{{STRUCTURE_2ab6| PDB=2ab6 | SCENE= }} | {{STRUCTURE_2ab6| PDB=2ab6 | SCENE= }} | ||
| - | + | ===HUMAN GLUTATHIONE S-TRANSFERASE M2-2 (E.C.2.5.1.18) complexed with S-METHYLGLUTATHIONE=== | |
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| - | ==Overview== | ||
| - | Human glutathione-S-transferase M2-2 (hGSTM2-2) was expressed in Escherichia coli and purified by GSH-affinity chromatography. The recombinant enzyme and the protein isolated from human tissue were indistinguishable based on physicochemical, enzymatic and immunological criteria. The catalytically active dimeric hGSTM2-2 was crystallized without GSH or other active-site ligands in two crystal forms. Diffraction from form A crystals extends to 2.5 A and is consistent with the space group P21 (a = 53.9, b = 81.5, c = 55.6 A, beta = 109.26 A) with two monomers in the asymmetric unit. Diffraction from form B crystals extends to 3 A and is consistent with a space group P212121 (a = 57.2, b = 80.7, c = 225.9 A) with two dimers in the asymmetric unit. This is the first report of ligand-free mu-class GST crystals, and a comparison with liganded complexes will provide insight into the structural consequences of substrate binding which are thought to be important for catalysis. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2AB6 is a | + | 2AB6 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB6 OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:9761928</ref><ref group="xtra">PMID:8182750</ref><ref group="xtra">PMID:2034681</ref><references group="xtra"/> | |
[[Category: Glutathione transferase]] | [[Category: Glutathione transferase]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Almo, S C.]] | [[Category: Almo, S C.]] | ||
[[Category: Listowsky, I.]] | [[Category: Listowsky, I.]] | ||
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[[Category: S-methylglutathione]] | [[Category: S-methylglutathione]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 06:30:23 2009'' | ||
Revision as of 04:30, 17 February 2009
HUMAN GLUTATHIONE S-TRANSFERASE M2-2 (E.C.2.5.1.18) complexed with S-METHYLGLUTATHIONE
About this Structure
2AB6 is a 4 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Patskovska LN, Fedorov AA, Patskovsky YV, Almo SC, Listowsky I. Expression, crystallization and preliminary X-ray analysis of ligand-free human glutathione S-transferase M2-2. Acta Crystallogr D Biol Crystallogr. 1998 May 1;54(Pt 3):458-60. PMID:9761928
- Raghunathan S, Chandross RJ, Kretsinger RH, Allison TJ, Penington CJ, Rule GS. Crystal structure of human class mu glutathione transferase GSTM2-2. Effects of lattice packing on conformational heterogeneity. J Mol Biol. 1994 May 20;238(5):815-32. PMID:8182750 doi:http://dx.doi.org/10.1006/jmbi.1994.1336
- Vorachek WR, Pearson WR, Rule GS. Cloning, expression, and characterization of a class-mu glutathione transferase from human muscle, the product of the GST4 locus. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4443-7. PMID:2034681
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