1mel
From Proteopedia
| Line 20: | Line 20: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1MEL is a | + | 1MEL is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MEL OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:8784355</ref><references group="xtra"/> | |
[[Category: Camelus dromedarius]] | [[Category: Camelus dromedarius]] | ||
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Desmyter, A.]] | [[Category: Desmyter, A.]] | ||
[[Category: Gharoudi, M Arbabi.]] | [[Category: Gharoudi, M Arbabi.]] | ||
| Line 38: | Line 37: | ||
[[Category: Camel single-domain anti-lysozyme]] | [[Category: Camel single-domain anti-lysozyme]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 06:34:41 2009'' |
Revision as of 04:34, 17 February 2009
| |||||||||
| 1mel, resolution 2.50Å () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Gene: | ANTIBODY VH FRAGMENT CAB-LYS3 (Camelus dromedarius), ANTIBODY VH FRAGMENT CAB-LYS3 (Gallus gallus) | ||||||||
| Activity: | Lysozyme, with EC number 3.2.1.17 | ||||||||
| |||||||||
| |||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
CRYSTAL STRUCTURE OF A CAMEL SINGLE-DOMAIN VH ANTIBODY FRAGMENT IN COMPLEX WITH LYSOZYME
The Camelidae is the only taxonomic family known to possess functional heavy-chain antibodies, lacking light chains. We report here the 2.5 A resolution crystal structure of a camel VH in complex with its antigen, lysozyme. Compared to human and mouse VH domains, there are no major backbone rearrangements in the VH framework. However, the architecture of the region of VH that interacts with a VL in a conventional FV is different from any previously seen. Moreover, the CDR1 region, although in sequence homologous to human CDR1, deviates fundamentally from the canonical structure. Additionally, one half of the CDR3 contacts the VH region which in conventional immunoglobulins interacts with a VL whereas the other half protrudes from the antigen binding site and penetrates deeply into the active site of lysozyme.
Crystal structure of a camel single-domain VH antibody fragment in complex with lysozyme., Desmyter A, Transue TR, Ghahroudi MA, Thi MH, Poortmans F, Hamers R, Muyldermans S, Wyns L, Nat Struct Biol. 1996 Sep;3(9):803-11. PMID:8784355
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1MEL is a 4 chains structure of sequences from Camelus dromedarius and Gallus gallus. Full crystallographic information is available from OCA.
Reference
- Desmyter A, Transue TR, Ghahroudi MA, Thi MH, Poortmans F, Hamers R, Muyldermans S, Wyns L. Crystal structure of a camel single-domain VH antibody fragment in complex with lysozyme. Nat Struct Biol. 1996 Sep;3(9):803-11. PMID:8784355
Page seeded by OCA on Tue Feb 17 06:34:41 2009
