2ayy
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(New page: 200px<br /><applet load="2ayy" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ayy" /> '''Solution structure of the E.coli RcsC C-term...)
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Revision as of 06:21, 21 November 2007
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Solution structure of the E.coli RcsC C-terminus (residues 700-816) containing linker region
Overview
The Rcs signalling pathway controls a variety of physiological functions, like capsule synthesis, cell division or motility in prokaryotes. The Rcs, regulation cascade, involving a multi-step phosphorelay between the two, membrane-bound hybrid sensor kinases RcsC and RcsD and the global, regulator RcsB, is, up to now, one of the most complicated regulatory, systems in bacteria. To understand the structural basis of Rcs signal, transduction, NMR spectroscopy was employed to determine the solution, structure of the RcsC C terminus, possessing a phosphoreceiver domain, (RcsC-PR), and a region previously described as a long linker between the, histidine kinase domain of RcsC (RcsC-HK) and the RcsC-PR. We have found, that the linker region comprises an independent structural domain of a new, alpha/beta organization, which we named RcsC-ABL domain (Alpha/Beta/Loop)., The ABL domain appears to be a conserved and unique structural element of, RcsC-like kinases with no significant sequence homology to other proteins., The second domain of the C terminus, the RcsC-PR domain, represents a, well-folded CheY-like phosphoreceiver domain with the central parallel, beta-sheet covered with two alpha-helical layers on both sides. We have, mapped the interaction of RcsC-ABL and RcsC-PR with the histidine, phosphotransfer domain (HPt) of RcsD. In addition we have characterized, the interaction with and the conformational effects of Mg2+ and the, phosphorylation mimetic BeF(-)(3) on RcsC-ABL and RcsC-PR.
About this Structure
2AYY is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
A new structural domain in the Escherichia coli RcsC hybrid sensor kinase connects histidine kinase and phosphoreceiver domains., Rogov VV, Rogova NY, Bernhard F, Koglin A, Lohr F, Dotsch V, J Mol Biol. 2006 Nov 17;364(1):68-79. Epub 2006 Jul 29. PMID:17005198
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