2b1o

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Solution Structure of Ca2+-bound DdCAD-1

Overview

DdCAD-1 is a novel Ca(2+)-dependent cell adhesion molecule that lacks a, hydrophobic signal peptide and a transmembrane domain. DdCAD-1 is, expressed by the social amoeba Dictyostelium discoideum at the onset of, development. It is synthesized as a soluble protein and then transported, to the plasma membrane by contractile vacuoles. Here we describe the novel, features of the solution structures of Ca(2+)-free and Ca(2+)-bound, monomeric DdCAD-1. DdCAD-1 contains two beta-sandwich domains, belonging, to the betagamma-crystallin and immunoglobulin fold classes, respectively., Whereas the N-terminal domain has a major role in homophilic binding, the, C-terminal domain tethers the protein to the cell membrane. From, structural and mutational analyses, we propose a model for the, Ca(2+)-bound DdCAD-1 dimer as a basis for understanding DdCAD-1-mediated, cell-cell adhesion at the molecular level. Our results provide new, insights into Ca(2+)-dependent mechanisms for cell-cell adhesion.

About this Structure

2B1O is a Single protein structure of sequence from Dictyostelium discoideum with CA as ligand. Full crystallographic information is available from OCA.

Reference

Solution structures of the adhesion molecule DdCAD-1 reveal new insights into Ca(2+)-dependent cell-cell adhesion., Lin Z, Sriskanthadevan S, Huang H, Siu CH, Yang D, Nat Struct Mol Biol. 2006 Nov;13(11):1016-22. Epub 2006 Oct 22. PMID:17057715

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