2b7v
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(New page: 200px<br /><applet load="2b7v" size="450" color="white" frame="true" align="right" spinBox="true" caption="2b7v" /> '''Structure of ADAR2 dsRBM2'''<br /> ==Overvi...)
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Revision as of 06:30, 21 November 2007
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Structure of ADAR2 dsRBM2
Overview
Adenosine deaminases that act on RNA (ADARs) site-selectively modify, adenosines to inosines within RNA transcripts, thereby recoding genomic, information. How ADARs select specific adenosine moieties for deamination, is poorly understood. Here, we report NMR structures of the two, double-stranded RNA binding motifs (dsRBMs) of rat ADAR2 and an NMR, chemical shift perturbation study of the interaction of the two dsRBMs, with a 71 nucleotide RNA encoding the R/G site of the GluR-B. We have, identified the protein and the RNA surfaces involved in complex formation, allowing us to present an NMR-based model of the complex. We have found, that dsRBM1 recognizes a conserved pentaloop, whereas dsRBM2 recognizes, two bulged bases adjacent to the editing site, demonstrating RNA, structure-dependent recognition by the ADAR2 dsRBMs. In vitro mutagenesis, studies with both the protein and the RNA further support our structural, findings.
About this Structure
2B7V is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Structure and specific RNA binding of ADAR2 double-stranded RNA binding motifs., Stefl R, Xu M, Skrisovska L, Emeson RB, Allain FH, Structure. 2006 Feb;14(2):345-55. PMID:16472753
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