This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2uun
From Proteopedia
(Difference between revisions)
(New page: 200px <!-- The line below this paragraph, containing "STRUCTURE_2uun", creates the "Structure Box" on the page. You may change the PDB parameter (which sets the PD...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2uun. | + | {{Seed}} |
| + | [[Image:2uun.png|left|200px]] | ||
<!-- | <!-- | ||
| Line 9: | Line 10: | ||
{{STRUCTURE_2uun| PDB=2uun | SCENE= }} | {{STRUCTURE_2uun| PDB=2uun | SCENE= }} | ||
| - | + | ===CRYSTAL STRUCTURE OF C-PHYCOCYANIN FROM PHORMIDIUM, LYNGBYA SPP. (MARINE) AND SPIRULINA SP. (FRESH WATER) SHOWS TWO DIFFERENT WAYS OF ENERGY TRANSFER BETWEEN TWO HEXAMERS.=== | |
| - | |||
| - | ==Overview== | ||
| - | C-phycocyanins from three cyanobacterial cultures of freshwater and marine habitat, Spirulina, Phormidium and Lyngbya spp., were purified to homogeneity and crystallized using the hanging-drop vapour-diffusion method. Blue-coloured crystals in different crystal forms, monoclinic and hexagonal, were obtained for the three species. The crystals took 1-12 weeks to grow to full size using polyethylene glycols of different molecular weights as precipitants. The amino-acid sequences of these proteins show high similarity to other known C-phycocyanins from related organisms; however, the C-phycocyanins reported here showed different biochemical and biophysical properties, i.e. molecular weight, stability etc. The X-ray diffraction data were collected at resolutions of 3.0 A for the monoclinic and 3.2 and 3.6 A for the hexagonal forms. The unit-cell parameters corresponding to the monoclinic space group P2(1) are a = 107.33, b = 115.64, c = 183.26 A, beta = 90.03 degrees for Spirulina sp. C-phycocyanin and are similar for crystals of Phormidium and Lyngbya spp. C-phycocyanins. Crystals belonging to the hexagonal space group P6(3), with unit-cell parameters a = b = 154.97, c = 40.35 A and a = b = 151.96, c = 39.06 A, were also obtained for the C-phycocyanins from Spirulina and Lyngbya spp., respectively. The estimated solvent content is around 50% for the monoclinic crystals of all three species assuming the presence of two hexamers per asymmetric unit. The solvent content is 66.5 and 64.1% for the hexagonal crystals of C-phycocyanin from Spirulina and Lyngbya spp. assuming the presence of one alphabeta monomer per asymmetric unit. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2UUN is a | + | 2UUN is a 24 chains structure of sequences from [http://en.wikipedia.org/wiki/Leptolyngbya_sp. Leptolyngbya sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UUN OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:16511175</ref><references group="xtra"/> | |
[[Category: Leptolyngbya sp.]] | [[Category: Leptolyngbya sp.]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Ghosh, P K.]] | [[Category: Ghosh, P K.]] | ||
[[Category: Mishra, S.]] | [[Category: Mishra, S.]] | ||
| Line 34: | Line 31: | ||
[[Category: Phormidium]] | [[Category: Phormidium]] | ||
[[Category: Spirulina sp]] | [[Category: Spirulina sp]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 08:50:33 2009'' | ||
Revision as of 06:50, 17 February 2009
CRYSTAL STRUCTURE OF C-PHYCOCYANIN FROM PHORMIDIUM, LYNGBYA SPP. (MARINE) AND SPIRULINA SP. (FRESH WATER) SHOWS TWO DIFFERENT WAYS OF ENERGY TRANSFER BETWEEN TWO HEXAMERS.
About this Structure
2UUN is a 24 chains structure of sequences from Leptolyngbya sp.. Full crystallographic information is available from OCA.
Reference
- Satyanarayana L, Suresh CG, Patel A, Mishra S, Ghosh PK. X-ray crystallographic studies on C-phycocyanins from cyanobacteria from different habitats: marine and freshwater. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Sep 1;61(Pt, 9):844-7. Epub 2005 Aug 31. PMID:16511175 doi:10.1107/S1744309105025649
Page seeded by OCA on Tue Feb 17 08:50:33 2009
