2bhg

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Revision as of 06:40, 21 November 2007

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spinqualitylabelsall models 2bhg, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3C PROTEASE FROM TYPE A10(61) FOOT-AND-MOUTH DISEASE VIRUS

Overview

Foot-and-mouth disease virus (FMDV) causes a widespread and economically, devastating disease of domestic livestock. Although FMDV vaccines are, available, political and technical problems associated with their use are, driving a renewed search for alternative methods of disease control. The, viral RNA genome is translated as a single polypeptide precursor that must, be cleaved into functional proteins by virally encoded proteases. 10 of, the 13 cleavages are performed by the highly conserved 3C protease, (3C(pro)), making the enzyme an attractive target for antiviral drugs. We, have developed a soluble, recombinant form of FMDV 3C(pro), determined the, crystal structure to 1.9-angstroms resolution, and analyzed the cleavage, specificity of the enzyme. The structure indicates that FMDV 3C(pro), adopts a chymotrypsin-like fold and possesses a Cys-His-Asp catalytic, triad in a similar conformation to the Ser-His-Asp triad conserved in, almost all serine proteases. This observation suggests that the dyad-based, mechanisms proposed for this class of cysteine proteases need to be, reassessed. Peptide cleavage assays revealed that the recognition sequence, spans at least four residues either side of the scissile bond (P4-P4') and, that FMDV 3C(pro) discriminates only weakly in favor of P1-Gln over, P1-Glu, in contrast to other 3C(pro) enzymes that strongly favor P1-Gln., The relaxed specificity may be due to the unexpected absence in FMDV, 3C(pro) of an extended beta-ribbon that folds over the substrate binding, cleft in other picornavirus 3C(pro) structures. Collectively, these, results establish a valuable framework for the development of FMDV 3C(pro), inhibitors.

About this Structure

2BHG is a Single protein structure of sequence from Foot-and-mouth disease virus. Active as Picornain 3C, with EC number 3.4.22.28 Full crystallographic information is available from OCA.

Reference

Crystal structure of foot-and-mouth disease virus 3C protease. New insights into catalytic mechanism and cleavage specificity., Birtley JR, Knox SR, Jaulent AM, Brick P, Leatherbarrow RJ, Curry S, J Biol Chem. 2005 Mar 25;280(12):11520-7. Epub 2005 Jan 14. PMID:15654079

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