2cxa

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Revision as of 07:11, 21 November 2007

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spinqualitylabelsall models 2cxa, resolution 1.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of Leucyl/phenylalanyl-tRNA protein transferase from Escherichia coli

Overview

Leucyl/phenylalanyl-tRNA-protein transferase (L/F-transferase) is an N-end, rule pathway enzyme, which catalyzes the transfer of Leu and Phe from, aminoacyl-tRNAs to exposed N-terminal Arg or Lys residues of acceptor, proteins. Here, we report the 1.6 A resolution crystal structure of, L/F-transferase (JW0868) from Escherichia coli, the first, three-dimensional structure of an L/F-transferase. The L/F-transferase, adopts a monomeric structure consisting of two domains that form a, bilobate molecule. The N-terminal domain forms a small lobe with a novel, fold. The large C-terminal domain has a highly conserved fold, which is, observed in the GCN5-related N-acetyltransferase (GNAT) family. Most of, the conserved residues of L/F-transferase reside in the central cavity, which exists at the interface between the N-terminal and C-terminal, domains. A comparison of the structures of L/F-transferase and the, bacterial peptidoglycan synthase FemX, indicated a structural homology in, the C-terminal domain, and a similar domain interface region. Although the, peptidyltransferase function is shared between the two proteins, the, enzymatic mechanism would differ. The conserved residues in the central, cavity of L/F-transferase suggest that this region is important for the, enzyme catalysis.

About this Structure

2CXA is a Single protein structure of sequence from Escherichia coli. Active as Leucyltransferase, with EC number 2.3.2.6 Full crystallographic information is available from OCA.

Reference

The crystal structure of leucyl/phenylalanyl-tRNA-protein transferase from Escherichia coli., Dong X, Kato-Murayama M, Muramatsu T, Mori H, Shirouzu M, Bessho Y, Yokoyama S, Protein Sci. 2007 Mar;16(3):528-34. Epub 2007 Jan 22. PMID:17242373

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