2d3d
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(New page: 200px<br /><applet load="2d3d" size="450" color="white" frame="true" align="right" spinBox="true" caption="2d3d, resolution 1.60Å" /> '''crystal structure of...)
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Revision as of 07:17, 21 November 2007
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crystal structure of the RNA binding SAM domain of saccharomyces cerevisiae Vts1
Overview
The SAM domain of the Saccharomyces cerevisiae post-transcriptional, regulator Vts1 has a high affinity towards RNA hairpins containing a CUGGC, pentaloop. We present the 1.6 Angstroms X-ray crystal structure of the, Vts1 SAM domain in its unliganded state, and the NMR solution structure of, this domain in its RNA-bound state. Both structures reveal a canonical, five helix SAM domain flanked by additional secondary structural elements, at the N and C termini. The two structures are essentially identical, implying that no major structural rearrangements occur upon RNA binding., Amide chemical shift changes map the RNA-binding site to a shallow, basic, patch at the junction of helix alpha5 and the loop connecting helices, alpha1 and alpha2.
About this Structure
2D3D is a Single protein structure of sequence from Saccharomyces cerevisiae with CA as ligand. Full crystallographic information is available from OCA.
Reference
The NMR and X-ray structures of the Saccharomyces cerevisiae Vts1 SAM domain define a surface for the recognition of RNA hairpins., Aviv T, Amborski AN, Zhao XS, Kwan JJ, Johnson PE, Sicheri F, Donaldson LW, J Mol Biol. 2006 Feb 17;356(2):274-9. Epub 2005 Dec 7. PMID:16375924
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