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2e77

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{{STRUCTURE_2e77| PDB=2e77 | SCENE= }}
{{STRUCTURE_2e77| PDB=2e77 | SCENE= }}
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'''Crystal structure of L-lactate oxidase with pyruvate complex'''
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===Crystal structure of L-lactate oxidase with pyruvate complex===
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==Overview==
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L-Lactate oxidase (LOX) from Aerococcus viridans catalyzes the oxidation of L-lactate to pyruvate by the molecular oxygen and belongs to a large family of 2-hydroxy acid-dependent flavoenzymes. To investigate the interaction of LOX with pyruvate in structural details and understand the chemical mechanism of flavin-dependent L-lactate dehydrogenation, the LOX-pyruvate complex was crystallized and the crystal structure of the complex has been solved at a resolution of 1.90 Angstrom. One pyruvate molecule bound to the active site and located near N5 position of FMN for subunits, A, B, and D in the asymmetric unit, were identified. The pyruvate molecule is stabilized by the interaction of its carboxylate group with the side-chain atoms of Tyr40, Arg181, His265, and Arg268, and of its keto-oxygen atom with the side-chain atoms of Tyr146, Tyr215, and His265. The alpha-carbon of pyruvate is found to be 3.13 Angstrom from the N5 atom of FMN at an angle of 105.4 degrees from the flavin N5-N10 axis.
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The line below this paragraph, {{ABSTRACT_PUBMED_17517371}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 17517371 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17517371}}
==About this Structure==
==About this Structure==
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2E77 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aerococcus_viridans Aerococcus viridans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E77 OCA].
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2E77 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Aerococcus_viridans Aerococcus viridans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E77 OCA].
==Reference==
==Reference==
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Crystallographic study on the interaction of L-lactate oxidase with pyruvate at 1.9 Angstrom resolution., Li SJ, Umena Y, Yorita K, Matsuoka T, Kita A, Fukui K, Morimoto Y, Biochem Biophys Res Commun. 2007 Jul 13;358(4):1002-7. Epub 2007 May 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17517371 17517371]
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<ref group="xtra">PMID:17517371</ref><references group="xtra"/>
[[Category: Aerococcus viridans]]
[[Category: Aerococcus viridans]]
[[Category: Lactate 2-monooxygenase]]
[[Category: Lactate 2-monooxygenase]]
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[[Category: Single protein]]
 
[[Category: Morimoto, Y.]]
[[Category: Morimoto, Y.]]
[[Category: Fmn]]
[[Category: Fmn]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
[[Category: Tim barrel]]
[[Category: Tim barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 02:03:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 13:22:26 2009''

Revision as of 11:22, 17 February 2009

Image:2e77.png

Template:STRUCTURE 2e77

Crystal structure of L-lactate oxidase with pyruvate complex

Template:ABSTRACT PUBMED 17517371

About this Structure

2E77 is a 4 chains structure of sequences from Aerococcus viridans. Full crystallographic information is available from OCA.

Reference

  • Li SJ, Umena Y, Yorita K, Matsuoka T, Kita A, Fukui K, Morimoto Y. Crystallographic study on the interaction of L-lactate oxidase with pyruvate at 1.9 Angstrom resolution. Biochem Biophys Res Commun. 2007 Jul 13;358(4):1002-7. Epub 2007 May 11. PMID:17517371 doi:10.1016/j.bbrc.2007.05.021

Page seeded by OCA on Tue Feb 17 13:22:26 2009

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