2dfc
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(New page: 200px<br /><applet load="2dfc" size="450" color="white" frame="true" align="right" spinBox="true" caption="2dfc, resolution 1.19Å" /> '''Xylanase II from Tri...)
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Revision as of 07:27, 21 November 2007
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Xylanase II from Tricoderma reesei at 293K
Overview
An orthorhombic crystal of xylanase II from Trichoderma reesei was grown, in the presence of sodium iodide. Crystal structures at atomic resolution, were determined at 100 and 293 K. Protein molecules were aligned along a, crystallographic twofold screw axis, forming a helically extended, polymer-like chain mediated by an iodide ion. The iodide ion connected, main-chain peptide groups between two adjacent molecules by an, N-H...I-...H-N hydrogen-bond bridge, thus contributing to regulation of, the molecular arrangement and suppression of the rigid-body motion in the, crystal with high diffraction quality. The structure at 293 K showed, considerable thermal motion in the loop regions connecting the, beta-strands that form the active-site cleft. TLS model analysis of the, thermal motion and a comparison between this structure and that at 100 K, suggest that the fluctuation of these loop regions is attributable to the, hinge-like movement of the beta-strands.
About this Structure
2DFC is a Single protein structure of sequence from Hypocrea jecorina with IOD as ligand. Active as Endo-1,4-beta-xylanase, with EC number 3.2.1.8 Full crystallographic information is available from OCA.
Reference
Structure of an orthorhombic form of xylanase II from Trichoderma reesei and analysis of thermal displacement., Watanabe N, Akiba T, Kanai R, Harata K, Acta Crystallogr D Biol Crystallogr. 2006 Jul;62(Pt 7):784-92. Epub 2006, Jun 20. PMID:16790934
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