1z5u
From Proteopedia
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{{STRUCTURE_1z5u| PDB=1z5u | SCENE= }} | {{STRUCTURE_1z5u| PDB=1z5u | SCENE= }} | ||
| - | + | ===Crystal structure of S. typhimurium AphA complexed with cyclic-AMP=== | |
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| - | ==Overview== | ||
| - | The aphA gene of Salmonella enterica sv. Typhimurium strain MD6001 was cloned in the multicopy plasmid pBluescript SK(-). The recombinant AphA protein was purified to homogeneity. The protein crystallized in the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 112.4, b = 130.2, c = 139.6 A. Consistent with the self-rotation function, there are two tetramers in the asymmetric unit, indicating a solvent content of approximately 54%. The crystals are composed of biologically active AphA molecules. | ||
==About this Structure== | ==About this Structure== | ||
| - | 1Z5U is a | + | 1Z5U is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z5U OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:14501135</ref><references group="xtra"/> | |
[[Category: Acid phosphatase]] | [[Category: Acid phosphatase]] | ||
[[Category: Salmonella typhimurium]] | [[Category: Salmonella typhimurium]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Kumar, V.]] | [[Category: Kumar, V.]] | ||
[[Category: Makde, R D.]] | [[Category: Makde, R D.]] | ||
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[[Category: Cyclic-amp complex of apha protein]] | [[Category: Cyclic-amp complex of apha protein]] | ||
[[Category: Metalloenzyme]] | [[Category: Metalloenzyme]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 13:27:26 2009'' | ||
Revision as of 11:27, 17 February 2009
Crystal structure of S. typhimurium AphA complexed with cyclic-AMP
About this Structure
1Z5U is a 4 chains structure of sequences from Salmonella typhimurium. Full crystallographic information is available from OCA.
Reference
- Makde RD, Kumar V, Gupta GD, Jasti J, Singh TP, Mahajan SK. Expression, purification, crystallization and preliminary X-ray diffraction studies of recombinant class B non-specific acid phosphatase of Salmonella typhimurium. Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1849-52. Epub 2003, Sep 19. PMID:14501135
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