2dj5
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(New page: 200px<br /><applet load="2dj5" size="450" color="white" frame="true" align="right" spinBox="true" caption="2dj5, resolution 2.55Å" /> '''Crystal Structure of...)
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Revision as of 07:30, 21 November 2007
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Crystal Structure of the vitamin B12 biosynthetic cobaltochelatase, CbiXS, from Archaeoglobus fulgidus
Overview
The Archaeoglobus fulgidus gene af0721 encodes CbiX(S), a small, cobaltochelatase associated with the anaerobic biosynthesis of vitamin B12, (cobalamin). The protein was shown to have activity both in vivo and in, vitro, catalyzing the insertion of Co2+ into sirohydrochlorin. The, structure of CbiX(S) was determined in two different crystal forms and was, shown to consist of a central mixed beta-sheet flanked by four, alpha-helices, one of which originates in the C-terminus of a neighboring, molecule. CbiX(S) is about half the size of other Class II tetrapyrrole, chelatases. The overall topography of CbiX(S) exhibits substantial, resemblance to both the N- and C-terminal regions of several members of, the Class II metal chelatases involved in tetrapyrrole biosynthesis. Two, histidines (His10 and His74), are in similar positions as the catalytic, histidine residues in the anaerobic cobaltochelatase CbiK (His145 and, His207). In light of the hypothesis that suggests the larger chelatases, evolved via gene duplication and fusion from a CbiX(S)-like enzyme, the, structure of AF0721 may represent that of an "ancestral" precursor of, class II metal chelatases.
About this Structure
2DJ5 is a Single protein structure of sequence from Archaeoglobus fulgidus with PO4 and GOL as ligands. Active as Sirohydrochlorin cobaltochelatase, with EC number 4.99.1.3 Full crystallographic information is available from OCA.
Reference
Crystal structure of the vitamin B12 biosynthetic cobaltochelatase, CbiXS, from Archaeoglobus fulgidus., Yin J, Xu LX, Cherney MM, Raux-Deery E, Bindley AA, Savchenko A, Walker JR, Cuff ME, Warren MJ, James MN, J Struct Funct Genomics. 2006 Mar;7(1):37-50. Epub 2006 Jul 12. PMID:16835730
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