2e5l

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Revision as of 07:50, 21 November 2007

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spinqualitylabelsall models 2e5l, resolution 3.300Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine- Dalgarno interaction

Overview

In the initiation phase of bacterial translation, the 30S ribosomal, subunit captures mRNA in preparation for binding with initiator tRNA. The, purine-rich Shine-Dalgarno (SD) sequence, in the 5' untranslated region of, the mRNA, anchors the 30S subunit near the start codon, via base pairing, with an anti-SD (aSD) sequence at the 3' terminus of 16S rRNA. Here, we, present the 3.3 A crystal structure of the Thermus thermophilus 30S, subunit bound with an mRNA mimic. The duplex formed by the SD and aSD, sequences is snugly docked in a "chamber" between the head and platform, domains, demonstrating how the 30S subunit captures and stabilizes the, otherwise labile SD helix. This location of the SD helix is suitable for, the placement of the start codon AUG in the immediate vicinity of the mRNA, channel, in agreement with reported crosslinks between the second position, of the start codon and G1530 of 16S rRNA.

About this Structure

2E5L is a Protein complex structure of sequences from Thermus thermophilus with ZN as ligand. Full crystallographic information is available from OCA.

Reference

A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine-Dalgarno interaction., Kaminishi T, Wilson DN, Takemoto C, Harms JM, Kawazoe M, Schluenzen F, Hanawa-Suetsugu K, Shirouzu M, Fucini P, Yokoyama S, Structure. 2007 Mar;15(3):289-97. PMID:17355865

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