3c0m
From Proteopedia
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{{STRUCTURE_3c0m| PDB=3c0m | SCENE= }} | {{STRUCTURE_3c0m| PDB=3c0m | SCENE= }} | ||
| - | + | ===Crystal structure of the proaerolysin mutant Y221G=== | |
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| - | ==Overview== | ||
| - | Aerolysin is chiefly responsible for the pathogenicity of Aeromonas hydrophila, a bacterium associated with diarrhoeal diseases and deep wound infections. Like many other microbial toxins, the protein changes in a multistep process from a completely water-soluble form to produce a transmembrane channel that destroys sensitive cells by breaking their permeability barriers. Here we describe the structure of proaerolysin determined by X-ray crystallography at 2.8 A resolution. The protoxin (M(r) 52,000) adopts a novel protein fold. Images of an aerolysin oligomer derived from electron microscopy have assisted in constructing a model of the membrane channel and have led to the proposal of a scheme to account for insertion of the protein into lipid bilayers to form ion channels. | ||
==About this Structure== | ==About this Structure== | ||
| - | 3C0M is a | + | 3C0M is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Aeromonas_hydrophila Aeromonas hydrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C0M OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:7510043</ref><ref group="xtra">PMID:2329576</ref><references group="xtra"/> | |
[[Category: Aeromonas hydrophila]] | [[Category: Aeromonas hydrophila]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Burr, S E.]] | [[Category: Burr, S E.]] | ||
[[Category: Goot, G van der.]] | [[Category: Goot, G van der.]] | ||
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[[Category: Secreted]] | [[Category: Secreted]] | ||
[[Category: Toxin]] | [[Category: Toxin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 16:22:35 2009'' | ||
Revision as of 14:22, 17 February 2009
Crystal structure of the proaerolysin mutant Y221G
About this Structure
3C0M is a 2 chains structure of sequences from Aeromonas hydrophila. Full crystallographic information is available from OCA.
Reference
- Parker MW, Buckley JT, Postma JP, Tucker AD, Leonard K, Pattus F, Tsernoglou D. Structure of the Aeromonas toxin proaerolysin in its water-soluble and membrane-channel states. Nature. 1994 Jan 20;367(6460):292-5. PMID:7510043 doi:http://dx.doi.org/10.1038/367292a0
- Tucker AD, Parker MW, Tsernoglou D, Buckley JT. Crystallization of a proform of aerolysin, a hole-forming toxin from Aeromonas hydrophila. J Mol Biol. 1990 Apr 20;212(4):561-2. PMID:2329576
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