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2hcy
From Proteopedia
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{{STRUCTURE_2hcy| PDB=2hcy | SCENE= }} | {{STRUCTURE_2hcy| PDB=2hcy | SCENE= }} | ||
| - | + | ===Yeast Alcohol Dehydrogenase I, Saccharomyces cerevisiae fermentative enzyme=== | |
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| - | ==Overview== | ||
| - | The cytoplasmic yeast alcohol dehydrogenase I crystallized at 5 degrees C as hexagonal plates or short columns in the presence of NAD+ and 2,2,2-trifluoroethanol, in sodium N-tris(hydroxymethyl)methyl-3-aminopropanesulfonate buffer at pH 8.2 to 8.6, using polyethylene glycol 4000 as precipitant. X-ray diffraction data to 3.2 A resolution show that the crystals are hexagonal in space group P622 with unit cell dimensions a = b = 147.9 A, c = 69.1 A. There is one subunit of the tetrameric enzyme per asymmetric unit, giving a packing density of 2.9 A3/Da. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2HCY is a | + | 2HCY is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HCY OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:8289298</ref><ref group="xtra">PMID:3546317</ref><references group="xtra"/> | |
[[Category: Alcohol dehydrogenase]] | [[Category: Alcohol dehydrogenase]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Plapp, B V.]] | [[Category: Plapp, B V.]] | ||
[[Category: Ramaswamy, S.]] | [[Category: Ramaswamy, S.]] | ||
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[[Category: Tetramer of asymmetric dimer]] | [[Category: Tetramer of asymmetric dimer]] | ||
[[Category: Zinc coordination]] | [[Category: Zinc coordination]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 17:22:04 2009'' | ||
Revision as of 15:22, 17 February 2009
Yeast Alcohol Dehydrogenase I, Saccharomyces cerevisiae fermentative enzyme
About this Structure
2HCY is a 4 chains structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
- Ramaswamy S, Kratzer DA, Hershey AD, Rogers PH, Arnone A, Eklund H, Plapp BV. Crystallization and preliminary crystallographic studies of Saccharomyces cerevisiae alcohol dehydrogenase I. J Mol Biol. 1994 Jan 14;235(2):777-9. PMID:8289298 doi:http://dx.doi.org/10.1006/jmbi.1994.1031
- Ganzhorn AJ, Green DW, Hershey AD, Gould RM, Plapp BV. Kinetic characterization of yeast alcohol dehydrogenases. Amino acid residue 294 and substrate specificity. J Biol Chem. 1987 Mar 15;262(8):3754-61. PMID:3546317
Page seeded by OCA on Tue Feb 17 17:22:04 2009
