2oqc
From Proteopedia
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{{STRUCTURE_2oqc| PDB=2oqc | SCENE= }} | {{STRUCTURE_2oqc| PDB=2oqc | SCENE= }} | ||
| - | + | ===Crystal Structure of Penicillin V acylase from Bacillus subtilis=== | |
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| - | ==Overview== | ||
| - | Penicillin acylase proteins are amidohydrolase enzymes that cleave penicillins at the amide bond connecting the side chain to their beta-lactam nucleus. An unannotated protein from Bacillus subtilis has been expressed in Escherichia coli, purified and confirmed to possess penicillin V acylase activity. The protein was crystallized using the hanging-drop vapour-diffusion method from a solution containing 4 M sodium formate in 100 mM Tris-HCl buffer pH 8.2. Diffraction data were collected under cryogenic conditions to a spacing of 2.5 A. The crystals belonged to the orthorhombic space group C222(1), with unit-cell parameters a = 111.0, b = 308.0, c = 56.0 A. The estimated Matthews coefficient was 3.23 A3 Da(-1), corresponding to 62% solvent content. The structure has been solved using molecular-replacement methods with B. sphaericus penicillin V acylase (PDB code 2pva) as the search model. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2OQC is a | + | 2OQC is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQC OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:16511127</ref><references group="xtra"/> | |
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Penicillin amidase]] | [[Category: Penicillin amidase]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Brannigan, J A.]] | [[Category: Brannigan, J A.]] | ||
[[Category: Dodson, G G.]] | [[Category: Dodson, G G.]] | ||
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[[Category: Ntn-hydrolase]] | [[Category: Ntn-hydrolase]] | ||
[[Category: Penicillin v acylase]] | [[Category: Penicillin v acylase]] | ||
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| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 17:59:48 2009'' | ||
Revision as of 15:59, 17 February 2009
Crystal Structure of Penicillin V acylase from Bacillus subtilis
About this Structure
2OQC is a 2 chains structure of sequences from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
- Rathinaswamy P, Pundle AV, Prabhune AA, Sivaraman H, Brannigan JA, Dodson GG, Suresh CG. Cloning, purification, crystallization and preliminary structural studies of penicillin V acylase from Bacillus subtilis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jul 1;61(Pt, 7):680-3. Epub 2005 Jun 15. PMID:16511127 doi:10.1107/S1744309105017987
Page seeded by OCA on Tue Feb 17 17:59:48 2009
