2ea1
From Proteopedia
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{{STRUCTURE_2ea1| PDB=2ea1 | SCENE= }} | {{STRUCTURE_2ea1| PDB=2ea1 | SCENE= }} | ||
| - | + | ===Crystal structure of Ribonuclease I from Escherichia coli COMPLEXED WITH GUANYLYL-2(PRIME),5(PRIME)-GUANOSINE=== | |
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| - | ==Overview== | ||
| - | We have constructed a strain that overproduces ribonuclease I of Escherichia coli and we have purified large quantities of the enzyme. Data from fluorescence, CD, and DSC measurements showed that it was a very stable protein. The conformation energy determined from urea and guanidine hydrochloride denaturation experiments was 11.5 kcal mol(-1) at pH 7.5. Thermal denaturation studies indicated that it had a T(m) of 64 degrees C at pH 4.0. RNase I belongs to the RNase T2/S-RNase group of endoribonucleases, but near the amino terminus it has an unusually long hydrophilic segment. Part of this was removed in the deletion construct, RNase I Delta(26-38). We have obtained crystals of both RNase I and of an enzyme-G2'p5'G complex in the P2(1) space group and oligonucleotide complexes with both wild type and mutant enzymes. The current study lays the groundwork for extensive investigation into the structure, function, and physical properties of this widely distributed group of ribonucleases. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2EA1 is a | + | 2EA1 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EA1 OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:11368513</ref><ref group="xtra">PMID:2541256</ref><references group="xtra"/> | |
[[Category: Enterobacter ribonuclease]] | [[Category: Enterobacter ribonuclease]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Lim, L W.]] | [[Category: Lim, L W.]] | ||
[[Category: Lim, R W.]] | [[Category: Lim, R W.]] | ||
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[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Protein-gpg complex]] | [[Category: Protein-gpg complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 19:32:05 2009'' | ||
Revision as of 17:32, 17 February 2009
Crystal structure of Ribonuclease I from Escherichia coli COMPLEXED WITH GUANYLYL-2(PRIME),5(PRIME)-GUANOSINE
About this Structure
2EA1 is a 1 chain structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
- Padmanabhan S, Zhou K, Chu CY, Lim RW, Lim LW. Overexpression, biophysical characterization, and crystallization of ribonuclease I from Escherichia coli, a broad-specificity enzyme in the RNase T2 family. Arch Biochem Biophys. 2001 Jun 1;390(1):42-50. PMID:11368513 doi:10.1006/abbi.2001.2359
- Koepke J, Maslowska M, Heinemann U, Saenger W. Three-dimensional structure of ribonuclease T1 complexed with guanylyl-2',5'-guanosine at 1.8 A resolution. J Mol Biol. 1989 Apr 5;206(3):475-88. PMID:2541256
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