2uza
From Proteopedia
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{{STRUCTURE_2uza| PDB=2uza | SCENE= }} | {{STRUCTURE_2uza| PDB=2uza | SCENE= }} | ||
| - | + | ===CRYSTAL STRUCTURE OF THE FREE RADICAL INTERMEDIATE OF PYRUVATE:FERREDOXIN OXIDOREDUCTASE FROM DESULFOVIBRIO AFRICANUS=== | |
| - | |||
| - | ==Overview== | ||
| - | Pyruvate-ferredoxin oxidoreductases (PFOR) are unique among thiamine pyrophosphate (ThDP)-containing enzymes in giving rise to a rather stable cofactor-based free-radical species upon the decarboxylation of their first substrate, pyruvate. We have obtained snapshots of unreacted and partially reacted (probably as a tetrahedral intermediate) pyruvate-PFOR complexes at different time intervals. We conclude that pyruvate decarboxylation involves very limited substrate-to-product movements but a significant displacement of the thiazolium moiety of ThDP. In this respect, PFOR seems to differ substantially from other ThDP-containing enzymes, such as transketolase and pyruvate decarboxylase. In addition, exposure of PFOR to oxygen in the presence of pyruvate results in significant inhibition of catalytic activity, both in solution and in the crystals. Examination of the crystal structure of inhibited PFOR suggests that the loss of activity results from oxime formation at the 4' amino substituent of the pyrimidine moiety of ThDP. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2UZA is a | + | 2UZA is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Desulfovibrio_africanus Desulfovibrio africanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UZA OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:16472741</ref><references group="xtra"/> | |
[[Category: Desulfovibrio africanus]] | [[Category: Desulfovibrio africanus]] | ||
[[Category: Pyruvate synthase]] | [[Category: Pyruvate synthase]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Cavazza, C.]] | [[Category: Cavazza, C.]] | ||
[[Category: Chabriere, E.]] | [[Category: Chabriere, E.]] | ||
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[[Category: Tpp-dependent enzyme]] | [[Category: Tpp-dependent enzyme]] | ||
[[Category: Transport]] | [[Category: Transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 20:57:15 2009'' | ||
Revision as of 18:57, 17 February 2009
CRYSTAL STRUCTURE OF THE FREE RADICAL INTERMEDIATE OF PYRUVATE:FERREDOXIN OXIDOREDUCTASE FROM DESULFOVIBRIO AFRICANUS
About this Structure
2UZA is a 2 chains structure of sequences from Desulfovibrio africanus. Full crystallographic information is available from OCA.
Reference
- Cavazza C, Contreras-Martel C, Pieulle L, Chabriere E, Hatchikian EC, Fontecilla-Camps JC. Flexibility of thiamine diphosphate revealed by kinetic crystallographic studies of the reaction of pyruvate-ferredoxin oxidoreductase with pyruvate. Structure. 2006 Feb;14(2):217-24. PMID:16472741 doi:10.1016/j.str.2005.10.013
Page seeded by OCA on Tue Feb 17 20:57:15 2009
Categories: Desulfovibrio africanus | Pyruvate synthase | Cavazza, C. | Chabriere, E. | Contreras-Martel, C. | Fontecilla-Camps, J C. | 4fe-4 | Electron transport | Iron | Iron-sulfur | Iron-sulfur cluster | Metal-binding | Oxidoreductase | Pyruvate | Pyruvate catabolism | Tpp-dependent enzyme | Transport
