2fv4
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(New page: 200px<br /><applet load="2fv4" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fv4" /> '''NMR solution structure of the yeast kinetoch...)
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Revision as of 08:40, 21 November 2007
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NMR solution structure of the yeast kinetochore Spc24/Spc25 globular domain
Overview
The Ndc80 complex, a kinetochore component conserved from yeast to humans, is essential for proper chromosome alignment and segregation during, mitosis. It is an approximately 570 A long, rod-shaped assembly of four, proteins--Ndc80p (Hec1), Nuf2p, Spc24p, and Spc25p--with globular regions, at either end of a central shaft. The complex bridges from the, centromere-proximal inner kinetochore layer at its Spc24/Spc25 globular, end to the microtubule binding outer kinetochore layer at its Ndc80/Nuf2, globular end. We report the atomic structures of the Spc24/Spc25 globular, domain, determined both by X-ray crystallography at 1.9 A resolution and, by NMR. Spc24 and Spc25 fold tightly together into a single globular, entity with pseudo-2-fold symmetry. Conserved residues line a common, hydrophobic core and the bottom of a cleft, indicating that the functional, orthologs from other eukaryotes will have the same structure and, suggesting a docking site for components of the inner kinetochore.
About this Structure
2FV4 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure of a central component of the yeast kinetochore: the Spc24p/Spc25p globular domain., Wei RR, Schnell JR, Larsen NA, Sorger PK, Chou JJ, Harrison SC, Structure. 2006 Jun;14(6):1003-9. PMID:16765893
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