2g2s
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(New page: 200px<br /><applet load="2g2s" size="450" color="white" frame="true" align="right" spinBox="true" caption="2g2s, resolution 1.20Å" /> '''Structure of S65G Y6...)
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Revision as of 08:47, 21 November 2007
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Structure of S65G Y66S GFP variant after spontaneous peptide hydrolysis
Overview
The green fluorescent protein (GFP) creates a fluorophore out of three, sequential amino acids by promoting spontaneous posttranslational, modifications. Here, we use high-resolution crystallography to, characterize GFP variants that not only undergo peptide backbone, cyclization but additional denaturation-induced peptide backbone, fragmentation, native peptide hydrolysis, and decarboxylation reactions., Our analyses indicate that architectural features that favor GFP peptide, cyclization also drive peptide hydrolysis. These results are relevant for, the maturation pathways of GFP homologues, such as the kindling, fluorescent protein and the Kaede protein, which use backbone cleavage to, red-shift the spectral properties of their chromophores. We further, propose a photochemical mechanism for the decarboxylation reaction, supporting a role for the GFP protein environment in facilitating radical, formation and one-electron chemistry, which may be important in activating, oxygen for the oxidation step of chromophore biosynthesis. Together, our, results characterize GFP posttranslational modification chemistry with, implications for the energetic landscape of backbone cyclization and, subsequent reactions, and for the rational design of predetermined, spontaneous backbone cyclization and cleavage reactions.
About this Structure
2G2S is a Protein complex structure of sequences from Aequorea victoria with MG as ligand. Full crystallographic information is available from OCA.
Reference
Understanding GFP posttranslational chemistry: structures of designed variants that achieve backbone fragmentation, hydrolysis, and decarboxylation., Barondeau DP, Kassmann CJ, Tainer JA, Getzoff ED, J Am Chem Soc. 2006 Apr 12;128(14):4685-93. PMID:16594705
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