1e3d

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spinqualitylabelsall models 1e3d, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

[NIFE] HYDROGENASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774

Overview

The primary and three-dimensional structures of a [NiFe] hydrogenase, isolated from D. desulfitricans ATCC 27774 were determined, by nucleotide, analysis and single-crystal X-ray crystallography. The three-dimensional, structural model was refined to R=0.167 and Rfree=0.223 using data to 1.8, A resolution. Two unique structural features are observed: the [4Fe-4S], cluster nearest the [NiFe] centre has been modified [4Fe-3S-3O] by loss of, one sulfur atom and inclusion of three oxygen atoms; a three-fold disorder, was observed for Cys536 which binds to the nickel atom in the [NiFe], centre. Also, the bridging sulfur atom that caps the active site was found, to have partial occupancy, thus corresponding to a partly activated, enzyme. These structural features may have biological relevance. ... [(full description)]

About this Structure

1E3D is a [Protein complex] structure of sequences from [Desulfovibrio desulfuricans] with MG, F3S, SF4, FSX, FNE and H2S as [ligands]. Full crystallographic information is available from [OCA].

Reference

[NiFe] hydrogenase from Desulfovibrio desulfuricans ATCC 27774: gene sequencing, three-dimensional structure determination and refinement at 1.8 A and modelling studies of its interaction with the tetrahaem cytochrome c3., Matias PM, Soares CM, Saraiva LM, Coelho R, Morais J, Le Gall J, Carrondo MA, J Biol Inorg Chem. 2001 Jan;6(1):63-81. PMID:11191224

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