2gek
From Proteopedia
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{{STRUCTURE_2gek| PDB=2gek | SCENE= }} | {{STRUCTURE_2gek| PDB=2gek | SCENE= }} | ||
| - | + | ===Crystal Structure of phosphatidylinositol mannosyltransferase (PimA) from Mycobacterium smegmatis in complex with GDP=== | |
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| - | ==Overview== | ||
| - | Phosphatidylinositol mannosyltransferase (PimA) is an essential enzyme for mycobacterial growth that catalyses the first mannosylation step in phosphatidyl-myo-inositol mannoside (PIM) biosynthesis. The enzyme belongs to the large GT4 family of glycosyltransferases, for which no structure is currently available. Recombinant purified PimA from Mycobacterium smegmatis has been crystallized in the presence of GDP and myo-inositol. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 37.2, b = 72.4, c = 138.2 A, and diffract to 2.4 A resolution. | ||
==About this Structure== | ==About this Structure== | ||
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GEK OCA]. | + | 2GEK is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GEK OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:16511084</ref><ref group="xtra">PMID:12068013</ref><ref group="xtra">PMID:15228522</ref><ref group="xtra">PMID:12765785</ref><ref group="xtra">PMID:12691742</ref><references group="xtra"/> | |
| + | [[Category: Mycobacterium smegmatis]] | ||
[[Category: Alzari, P M.]] | [[Category: Alzari, P M.]] | ||
[[Category: Buschiazzo, A.]] | [[Category: Buschiazzo, A.]] | ||
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[[Category: Mannosyltransferase]] | [[Category: Mannosyltransferase]] | ||
[[Category: Rossmann fold]] | [[Category: Rossmann fold]] | ||
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| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 02:50:39 2009'' | ||
Revision as of 00:50, 18 February 2009
Crystal Structure of phosphatidylinositol mannosyltransferase (PimA) from Mycobacterium smegmatis in complex with GDP
About this Structure
2GEK is a 1 chain structure of sequence from Mycobacterium smegmatis. Full crystallographic information is available from OCA.
Reference
- Guerin ME, Buschiazzo A, Kordulakova J, Jackson M, Alzari PM. Crystallization and preliminary crystallographic analysis of PimA, an essential mannosyltransferase from Mycobacterium smegmatis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 May 1;61(Pt, 5):518-20. Epub 2005 Apr 22. PMID:16511084 doi:10.1107/S1744309105012364
- Kordulakova J, Gilleron M, Mikusova K, Puzo G, Brennan PJ, Gicquel B, Jackson M. Definition of the first mannosylation step in phosphatidylinositol mannoside synthesis. PimA is essential for growth of mycobacteria. J Biol Chem. 2002 Aug 30;277(35):31335-44. Epub 2002 Jun 14. PMID:12068013 doi:10.1074/jbc.M204060200
- Briken V, Porcelli SA, Besra GS, Kremer L. Mycobacterial lipoarabinomannan and related lipoglycans: from biogenesis to modulation of the immune response. Mol Microbiol. 2004 Jul;53(2):391-403. PMID:15228522 doi:10.1111/j.1365-2958.2004.04183.x
- Nigou J, Gilleron M, Puzo G. Lipoarabinomannans: from structure to biosynthesis. Biochimie. 2003 Jan-Feb;85(1-2):153-66. PMID:12765785
- Coutinho PM, Deleury E, Davies GJ, Henrissat B. An evolving hierarchical family classification for glycosyltransferases. J Mol Biol. 2003 Apr 25;328(2):307-17. PMID:12691742
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