2gsy
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(New page: 200px<br /><applet load="2gsy" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gsy, resolution 2.60Å" /> '''The 2.6A structure o...)
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Revision as of 09:15, 21 November 2007
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The 2.6A structure of Infectious Bursal Virus Derived T=1 Particles
Overview
Infectious bursal disease virus (IBDV), a member of the Birnaviridae, family, is a double-stranded RNA virus that causes a highly contagious, disease in young chickens leading to significant economic losses in the, poultry industry. The VP2 protein, the only structural component of the, IBDV icosahedral capsid, spontaneously assembles into T=1 subviral, particles (SVP) when individually expressed as a chimeric gene. We have, determined the crystal structure of the T=1 SVP to 2.60 A resolution. Our, results show that the 20 trimeric VP2 clusters forming the T=1 shell are, further stabilized by calcium ions located at the threefold icosahedral, axes. The structure also reveals a new unexpected domain swapping that, mediates interactions between adjacent trimers: a short helical segment, located close to the end of the long C-terminal arm of VP2 is projected, toward the threefold axis of a neighboring VP2 trimer, leading to a, complex network of interactions that increases the stability of the T=1, particles. Analysis of crystal packing shows that the exposed capsid, residues, His253 and Thr284, determinants of IBDV virulence and the, adaptation of the virus to grow in cell culture, are involved in, particle-particle interactions.
About this Structure
2GSY is a Single protein structure of sequence from Infectious bursal disease virus with CA as ligand. Full crystallographic information is available from OCA.
Reference
The 2.6-Angstrom structure of infectious bursal disease virus-derived T=1 particles reveals new stabilizing elements of the virus capsid., Garriga D, Querol-Audi J, Abaitua F, Saugar I, Pous J, Verdaguer N, Caston JR, Rodriguez JF, J Virol. 2006 Jul;80(14):6895-905. PMID:16809295
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