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2j89
From Proteopedia
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(New page: 200px<br /> <applet load="2j89" size="450" color="white" frame="true" align="right" spinBox="true" caption="2j89, resolution 1.70Å" /> '''FUNCTIONAL AND STRU...)
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Revision as of 19:58, 29 October 2007
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FUNCTIONAL AND STRUCTURAL ASPECTS OF POPLAR CYTOSOLIC AND PLASTIDIAL TYPE A METHIONINE SULFOXIDE REDUCTASES
Overview
The genome of Populus trichocarpa contains five methionine sulfoxide, reductase A genes. Here, both cytosolic (cMsrA) and plastidial (pMsrA), poplar MsrAs were analyzed. The two recombinant enzymes are active in the, reduction of methionine sulfoxide with either dithiothreitol or poplar, thioredoxin as a reductant. In both enzymes, five cysteines, at positions, 46, 81, 100, 196, and 202, are conserved. Biochemical and enzymatic, analyses of the cysteine-mutated MsrAs support a catalytic mechanism, involving three cysteines at positions 46, 196, and 202. Cys(46) is the, catalytic cysteine, and the two C-terminal cysteines, Cys(196) and, Cys(202), are implicated in the thioredoxin-dependent recycling mechanism., Inspection of the pMsrA x-ray three-dimensional structure, which has been, ... [(full description)]
About this Structure
2J89 is a [Single protein] structure of sequence from [Populus trichocarpa] with BME as [ligand]. Full crystallographic information is available from [OCA].
Reference
Functional and structural aspects of poplar cytosolic and plastidial type a methionine sulfoxide reductases., Rouhier N, Kauffmann B, Tete-Favier F, Palladino P, Gans P, Branlant G, Jacquot JP, Boschi-Muller S, J Biol Chem. 2007 Feb 2;282(5):3367-78. Epub 2006 Nov 29. PMID:17135266
Page seeded by OCA on Mon Oct 29 22:03:21 2007
