1wpu
From Proteopedia
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{{STRUCTURE_1wpu| PDB=1wpu | SCENE= }} | {{STRUCTURE_1wpu| PDB=1wpu | SCENE= }} | ||
| - | + | ===Crystal Structure of the HutP antitermination complex bound to a single stranded region of hut mRNA=== | |
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| - | ==Overview== | ||
| - | HutP is an L-histidine-activated RNA binding protein that regulates the expression of the histidine utilization (hut) operon in Bacillus subtilis by binding to cis-acting regulatory sequences on the hut mRNA. The crystal structure of HutP complexed with an L-histidine analog showed a novel fold; there are four antiparallel beta strands in the central region of each monomer, with two alpha helices each on the front and back. Two HutP monomers form a dimer, and three dimers are arranged in crystallographic 3-fold symmetry to form a hexamer. A histidine analog was located in between the two monomers of HutP, with the imidazole group of L-histidine hydrogen bonded to Glu81. An activation mechanism is proposed based on the identification of key residues of HutP. The HutP binding region in hut mRNA was defined: it consists of three UAG trinucleotide motifs separated by four spacer nucleotides. Residues of HutP potentially important for RNA binding were identified. | ||
==About this Structure== | ==About this Structure== | ||
| - | 1WPU is a | + | 1WPU is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WPU OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:15242603</ref><ref group="xtra">PMID:15273277</ref><references group="xtra"/> | |
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Kumar, P K.R.]] | [[Category: Kumar, P K.R.]] | ||
[[Category: Kumarevel, T S.]] | [[Category: Kumarevel, T S.]] | ||
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[[Category: Rna binding]] | [[Category: Rna binding]] | ||
[[Category: Transcription regulation]] | [[Category: Transcription regulation]] | ||
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| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 04:20:34 2009'' | ||
Revision as of 02:20, 18 February 2009
Crystal Structure of the HutP antitermination complex bound to a single stranded region of hut mRNA
About this Structure
1WPU is a 4 chains structure of sequences from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
- Kumarevel T, Fujimoto Z, Karthe P, Oda M, Mizuno H, Kumar PK. Crystal structure of activated HutP; an RNA binding protein that regulates transcription of the hut operon in Bacillus subtilis. Structure. 2004 Jul;12(7):1269-80. PMID:15242603 doi:http://dx.doi.org/10.1016/j.str.2004.05.005
- Kumarevel TS, Gopinath SC, Nishikawa S, Mizuno H, Kumar PK. Identification of important chemical groups of the hut mRNA for HutP interactions that regulate the hut operon in Bacillus subtilis. Nucleic Acids Res. 2004 Jul 25;32(13):3904-12. Print 2004. PMID:15273277 doi:http://dx.doi.org/10.1093/nar/gkh725
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