2h5z
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="2h5z" size="450" color="white" frame="true" align="right" spinBox="true" caption="2h5z, resolution 1.92Å" /> '''Crystallographic str...)
Next diff →
Revision as of 09:26, 21 November 2007
|
Crystallographic structure of digestive lysozyme 1 from Musca domestica bound to chitotetraose at 1.92 A resolution
Overview
Lysozymes are mostly known for their defensive role against bacteria, but, in several animals lysozymes have a digestive function. Here, the initial, crystallographic characterization of two digestive lysozymes from Musca, domestica are presented. The proteins were crystallized using the, sitting-drop vapour-diffusion method in the presence of ammonium sulfate, or PEG/2-propanol as the precipitant. X-ray diffraction data were, collected to a maximum resolution of 1.9 angstroms using synchrotron, radiation. The lysozyme 1 and 2 crystals belong to the monoclinic space, group P2(1) (unit-cell parameters a = 36.52, b = 79.44, c = 45.20, angstroms, beta = 102.97 degrees) and the orthorhombic space group, P2(1)2(1)2 (unit-cell parameters a = 73.90, b = 96.40, c = 33.27, angstroms), respectively. The crystal structures were solved by molecular, replacement and structure refinement is in progress.
About this Structure
2H5Z is a Single protein structure of sequence from Musca domestica with CTO as ligand. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
Reference
Crystallization, data collection and phasing of two digestive lysozymes from Musca domestica., Marana SR, Cancado FC, Valerio AA, Ferreira C, Terra WR, Barbosa JA, Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt, 8):750-2. Epub 2006 Jul 24. PMID:16880547
Page seeded by OCA on Wed Nov 21 11:33:26 2007
