2h6r
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(New page: 200px<br /><applet load="2h6r" size="450" color="white" frame="true" align="right" spinBox="true" caption="2h6r, resolution 2.30Å" /> '''Crystal Structure of...)
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Revision as of 09:26, 21 November 2007
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Crystal Structure of triosephosphate isomerase (TIM) from Methanocaldococcus jannaschii
Overview
The crystal structure of a recombinant triosephosphate isomerase (TIM), from the archaeabacterium Methanocaldococcus jannaschii has been, determined at a resolution of 2.3 A using X-ray diffraction data from a, tetartohedrally twinned crystal. M. jannaschii TIM (MjTIM) is tetrameric, as suggested by solution studies and from the crystal structure, as is the, case for two other structurally characterized archaeal TIMs. The, archaeabacterial TIMs are shorter compared with the dimeric TIMs; the, insertions in the dimeric TIMs occur in the vicinity of the tetramer, interface, resulting in a hindrance to tetramerization in the dimeric, TIMs. The charge distribution on the surface of the archaeal TIMs also, facilitates tetramerization. Analysis of the barrel interactions in TIMs, suggests that these interactions are unlikely to account for the thermal, stability of the archaeal TIMs. A novelty of the unliganded structure of, MjTIM is the complete absence of electron density for the loop 6 residues., The disorder of this loop could be ascribed to a missing salt bridge, between residues at the N- and C-terminal ends of the loop in MjTIM.
About this Structure
2H6R is a Single protein structure of sequence from Methanocaldococcus jannaschii. Active as Triose-phosphate isomerase, with EC number 5.3.1.1 Full crystallographic information is available from OCA.
Reference
Structure of triosephosphate isomerase (TIM) from Methanocaldococcus jannaschii., Gayathri P, Banerjee M, Vijayalakshmi A, Azeez S, Balaram H, Balaram P, Murthy MR, Acta Crystallogr D Biol Crystallogr. 2007 Feb;63(Pt 2):206-20. Epub 2007, Jan 16. PMID:17242514
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