2caj

From Proteopedia

Revision as of 20:03, 29 October 2007

NIKR FROM HELICOBACTER PYLORI IN CLOSED TRANS-CONFORMATION AND NICKEL BOUND TO 4 INTERMEDIARY SITES

Overview

The survival of Helicobacter pylori in the human stomach critically relies, on the availability and use of nickel, an absolute cofactor of the, important virulence determinant urease. Nickel-responsive gene regulation, is mediated by HpNikR, a protein belonging to the ribbon-helix-helix, family of transcriptional regulators. Unlike its homologues, HpNikR acts, as both a repressor and an activator within an acid adaptation cascade. We, report the crystal structure of the full-length HpNikR in a nickel-free, conformation and two nickel-bound structures obtained in different, conditions: Ni1-HpNikR and Ni2-HpNikR. Apo-HpNikR shows the same global, fold as its bacterial homologues although with an unusual closed, trans-conformation and asymmetrical quaternary arrangement. The structure, of ... [(full description)]

About this Structure

2CAJ is a [Single protein] structure of sequence from [Helicobacter pylori] with CL, NI and GOL as [ligands]. Full crystallographic information is available from [OCA].

Reference

Structural basis of the nickel response in Helicobacter pylori: crystal structures of HpNikR in Apo and nickel-bound states., Dian C, Schauer K, Kapp U, McSweeney SM, Labigne A, Terradot L, J Mol Biol. 2006 Aug 25;361(4):715-30. Epub 2006 Jul 7. PMID:16872629

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