2hzv

From Proteopedia

Revision as of 09:57, 21 November 2007

NikR-operator DNA complex

Overview

Metal ion homeostasis is critical to the survival of all cells. Regulation, of nickel concentrations in Escherichia coli is mediated by the NikR, repressor via nickel-induced transcriptional repression of the nickel, ABC-type transporter, NikABCDE. Here, we report two crystal structures of, nickel-activated E. coli NikR, the isolated repressor at 2.1 A resolution, and in a complex with its operator DNA sequence from the nik promoter at, 3.1 A resolution. Along with the previously published structure of, apo-NikR, these structures allow us to evaluate functional proposals for, how metal ions activate NikR, delineate the drastic conformational changes, required for operator recognition, and describe the formation of a second, metal-binding site in the presence of DNA. They also provide a rare set of, structural views of a ligand-responsive transcription factor in the, unbound, ligand-induced, and DNA-bound states, establishing a model system, for the study of ligand-mediated effects on transcription factor function.

About this Structure

2HZV is a Single protein structure of sequence from Escherichia coli with NI and K as ligands. Full crystallographic information is available from OCA.

Reference

NikR-operator complex structure and the mechanism of repressor activation by metal ions., Schreiter ER, Wang SC, Zamble DB, Drennan CL, Proc Natl Acad Sci U S A. 2006 Sep 12;103(37):13676-81. Epub 2006 Aug 31. PMID:16945905

Page seeded by OCA on Wed Nov 21 12:04:31 2007