2i91
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(New page: 200px<br /><applet load="2i91" size="450" color="white" frame="true" align="right" spinBox="true" caption="2i91, resolution 2.65Å" /> '''60kDa Ro autoantigen...)
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Revision as of 10:04, 21 November 2007
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60kDa Ro autoantigen in complex with a fragment of misfolded RNA
Overview
The Ro autoantigen is ring-shaped, binds misfolded noncoding RNAs and is, proposed to function in quality control. Here we determine how Ro, interacts with misfolded RNAs. Binding of Ro to misfolded precursor, (pre)-5S ribosomal RNA requires a single-stranded 3' end and helical, elements. As mutating most sequences of the helices and tail results in, modest decreases in binding, Ro may be able to associate with a range of, RNAs. Ro binds several other RNAs that contain single-stranded tails. A, crystal structure of Ro bound to a misfolded pre-5S rRNA fragment reveals, that the tail inserts into the cavity, while a helix binds on the surface., Most contacts of Ro with the helix are to the backbone. Mutagenesis, reveals that Ro has an extensive RNA-binding surface. We propose that Ro, uses this surface to scavenge RNAs that fail to bind their specific, RNA-binding proteins.
About this Structure
2I91 is a Single protein structure of sequence from Xenopus laevis with ACT and MG as ligands. Full crystallographic information is available from OCA.
Reference
Structural and biochemical basis for misfolded RNA recognition by the Ro autoantigen., Fuchs G, Stein AJ, Fu C, Reinisch KM, Wolin SL, Nat Struct Mol Biol. 2006 Nov;13(11):1002-9. Epub 2006 Oct 15. PMID:17041599
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