2an7

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{{STRUCTURE_2an7| PDB=2an7 | SCENE= }}
{{STRUCTURE_2an7| PDB=2an7 | SCENE= }}
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'''Solution structure of the bacterial antidote ParD'''
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===Solution structure of the bacterial antidote ParD===
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==Overview==
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NMR and CD spectroscopy have been used to characterize, both structurally and dynamically, the 82-amino-acid ParD protein of the post-segregational killing module of the broad-host-range plasmid RP4/RK2. ParD occurs as a dimer in solution and exercises two different control functions; an autoregulatory function by binding to its own promoter P(parDE) and a plasmid-stabilizing function by inhibiting ParE toxicity in cells that express ParD and ParE. Analysis of the secondary structure based on the chemical-shift indices, sequential nuclear Overhauser enhancements (NOEs) and (3)J(Halpha-NH) scalar coupling constants showed that the N-terminal domain of ParD consists of a short beta-ribbon followed by three alpha-helices, demonstrating that ParD contains a ribbon-helix-helix fold, a DNA-binding motif found in a family of small prokaryotic repressors. (15)N longitudinal (T(1)) and transverse (T(2)) relaxation measurements and hetero nuclear NOEs showed that ParD is divided into two separate domains, a well-ordered N-terminal domain and a very flexible C-terminal domain. An increase in secondary structure was observed upon addition of trifluoroethanol, suggested to result from the formation of structured stretches in the C-terminal part of the protein. This is the first experimental evidence that the DNA-binding domain of ParD belongs to the ribbon-helix-helix fold family, and this structural motif is proposed to be present in functionally similar antidote proteins.
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(as it appears on PubMed at http://www.pubmed.gov), where 17656583 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17656583}}
==About this Structure==
==About this Structure==
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2AN7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AN7 OCA].
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2AN7 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AN7 OCA].
==Reference==
==Reference==
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The anti-toxin ParD of plasmid RK2 consists of two structurally distinct moieties and belongs to the ribbon-helix-helix family of DNA-binding proteins., Oberer M, Zangger K, Prytulla S, Keller W, Biochem J. 2002 Jan 1;361(Pt 1):41-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11743881 11743881]
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<ref group="xtra">PMID:17656583</ref><ref group="xtra">PMID:11743881</ref><references group="xtra"/>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
 
[[Category: Gruber, K.]]
[[Category: Gruber, K.]]
[[Category: Keller, W.]]
[[Category: Keller, W.]]
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[[Category: Zangger, K.]]
[[Category: Zangger, K.]]
[[Category: Bacterial antidote]]
[[Category: Bacterial antidote]]
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[[Category: Dna binding protein]]
[[Category: Dna-binding motif]]
[[Category: Dna-binding motif]]
[[Category: Plasmid addiction]]
[[Category: Plasmid addiction]]
[[Category: Ribbon-helix-helix]]
[[Category: Ribbon-helix-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 25 08:15:52 2009''

Revision as of 06:15, 25 February 2009

Image:2an7.png

Template:STRUCTURE 2an7

Solution structure of the bacterial antidote ParD

Template:ABSTRACT PUBMED 17656583

About this Structure

2AN7 is a 2 chains structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

  • Oberer M, Zangger K, Gruber K, Keller W. The solution structure of ParD, the antidote of the ParDE toxin antitoxin module, provides the structural basis for DNA and toxin binding. Protein Sci. 2007 Aug;16(8):1676-88. PMID:17656583 doi:16/8/1676
  • Oberer M, Zangger K, Prytulla S, Keller W. The anti-toxin ParD of plasmid RK2 consists of two structurally distinct moieties and belongs to the ribbon-helix-helix family of DNA-binding proteins. Biochem J. 2002 Jan 1;361(Pt 1):41-7. PMID:11743881

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