Sandbox3

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I looked for the earliest GFP structure I could find in the protein data bank and found 1ema. I searched Proteopedia for 1ema, clicked "edit page" button at the top, and then copied the contents below.
I looked for the earliest GFP structure I could find in the protein data bank and found 1ema. I searched Proteopedia for 1ema, clicked "edit page" button at the top, and then copied the contents below.
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{{Seed}}
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[[Image:1ema.png|left|200px]]
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<!--
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The line below this paragraph, containing "STRUCTURE_1ema", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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-->
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{{STRUCTURE_1ema| PDB=1ema | SCENE= }}
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===GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA===
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<!--
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The line below this paragraph, {{ABSTRACT_PUBMED_8703075}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 8703075 is the PubMed ID number.
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-->
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{{ABSTRACT_PUBMED_8703075}}
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==About this Structure==
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1EMA is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. The June 2003 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Green Fluorescent Protein (GFP)'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2003_6 10.2210/rcsb_pdb/mom_2003_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EMA OCA].
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==Reference==
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<ref group="xtra">PMID:8703075</ref><references group="xtra"/>
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[[Category: Aequorea victoria]]
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[[Category: Ormo, M.]]
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[[Category: Remington, S J.]]
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[[Category: Autocatalytic]]
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[[Category: Beta-barrel]]
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[[Category: Bioluminescense fluorescent protein]]
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[[Category: Fluorophore]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 16:53:56 2009''
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and display another structure.
and display another structure.
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{{STRUCTURE_1ema | PDB=1ema | SCENE= }}
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{{STRUCTURE_3cin | PDB=3cin | SCENE= }}

Revision as of 23:29, 1 March 2009

This sandbox is in use until June 1, 2009 for UMass Chemistry 490a. Others please do not edit this page. Thanks!

L Thompson 3/1/09 Start by finding a protein with a known structure that interests you (see assignment sheet instructions). Determine whether anyone else in the class has already started a sandbox page for your protein (not sure yet how to do this easily...). If so, join their page. If not, start a new sandbox page.

Copy the message at the top into your sandbox page to "reserve" this sandbox for this course (others can still edit it but there is a way to go back -- not sure how yet).

Give "credit" for all your entries: add your name (like mine above) and also the source of anything that you got from another source.

Look of the pdb id for your protein in the Protein Data Bank. Use this code to find the Proteopedia entry: this is the "automatically seeded entry". Copy this into your sandbox as a starting point.

I looked for the earliest GFP structure I could find in the protein data bank and found 1ema. I searched Proteopedia for 1ema, clicked "edit page" button at the top, and then copied the contents below.


PDB ID 1ema

Drag the structure with the mouse to rotate
1ema, resolution 1.90Å ()
Non-Standard Residues: ,
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml



Contents

GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA

Template:ABSTRACT PUBMED 8703075

About this Structure

1EMA is a 1 chain structure of sequence from Aequorea victoria. The June 2003 RCSB PDB Molecule of the Month feature on Green Fluorescent Protein (GFP) by David S. Goodsell is 10.2210/rcsb_pdb/mom_2003_6. Full crystallographic information is available from OCA.

Reference

  • Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ. Crystal structure of the Aequorea victoria green fluorescent protein. Science. 1996 Sep 6;273(5280):1392-5. PMID:8703075

Page seeded by OCA on Tue Feb 17 16:53:56 2009


This is a placeholder

This is a placeholder text to help you get started in placing a Jmol applet on your page. At any time, click "Show Preview" at the bottom of this page to see how it goes.

Replace the PDB id (use lowercase!) after the STRUCTURE_ and after PDB= to load and display another structure.


PDB ID 3cin

Drag the structure with the mouse to rotate
3cin, resolution 1.70Å ()
Ligands: , ,
Gene: TM1419, TM_1419 (Thermotoga maritima MSB8)
Activity: Inositol-3-phosphate synthase, with EC number 5.5.1.4
Resources: FirstGlance, OCA, RCSB, PDBsum, TOPSAN
Coordinates: save as pdb, mmCIF, xml


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