2j04

From Proteopedia

Revision as of 10:23, 21 November 2007

THE TAU60-TAU91 SUBCOMPLEX OF YEAST TRANSCRIPTION FACTOR IIIC

Overview

Yeast RNA polymerase III is recruited upon binding of subcomplexes tauA, and tauB of transcription factor IIIC (TFIIIC) to the A and B blocks of, tRNA gene promoters. The tauB subcomplex consists of subunits tau60, tau91, and tau138. We determined the 3.2 A crystal structure of tau60, bound to a large C-terminal fragment of tau91 (Deltatau91). Deltatau91, protein contains a seven-bladed propeller preceded by an N-terminal, extension, whereas tau60 contains a structurally homologous propeller, followed by a C-terminal domain with a novel alpha/beta fold. The two, propeller domains do not have any detectable DNA binding activity and, mediate heterodimer formation that may serve as scaffold for tau138, assembly. We show that the C-terminal tau60 domain interacts with the TATA, binding protein (TBP). Recombinant tauB recruits TBP and stimulates, TFIIIB-directed transcription on a TATA box containing tRNA gene, implying, a combined contribution of tauA and tauB to preinitiation complex, formation.

About this Structure

2J04 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structure of the tau60/Delta tau91 subcomplex of yeast transcription factor IIIC: insights into preinitiation complex assembly., Mylona A, Fernandez-Tornero C, Legrand P, Haupt M, Sentenac A, Acker J, Muller CW, Mol Cell. 2006 Oct 20;24(2):221-32. PMID:17052456

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