2j5o
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(New page: 200px<br /><applet load="2j5o" size="450" color="white" frame="true" align="right" spinBox="true" caption="2j5o" /> '''PSEUDOMONAS AERUGINOSA FTSK GAMMA DOMAIN'''<...)
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Revision as of 10:25, 21 November 2007
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PSEUDOMONAS AERUGINOSA FTSK GAMMA DOMAIN
Overview
The bacterial septum-located DNA translocase FtsK coordinates circular, chromosome segregation with cell division. Rapid translocation of DNA by, FtsK is directed by 8-base-pair DNA motifs (KOPS), so that newly, replicated termini are brought together at the developing septum, thereby, facilitating completion of chromosome segregation. Translocase functions, reside in three domains, alpha, beta and gamma. FtsKalphabeta are, necessary and sufficient for ATP hydrolysis-dependent DNA translocation, which is modulated by FtsKgamma through its interaction with KOPS. By, solving the FtsKgamma structure by NMR, we show that gamma is a, winged-helix domain. NMR chemical shift mapping localizes the DNA-binding, site on the gamma domain. Mutated proteins with substitutions in the, FtsKgamma DNA-recognition helix are impaired in DNA binding and KOPS, recognition, yet remain competent in DNA translocation and XerCD-dif, site-specific recombination, which facilitates the late stages of, chromosome segregation.
About this Structure
2J5O is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
Reference
The FtsK gamma domain directs oriented DNA translocation by interacting with KOPS., Sivanathan V, Allen MD, de Bekker C, Baker R, Arciszewska LK, Freund SM, Bycroft M, Lowe J, Sherratt DJ, Nat Struct Mol Biol. 2006 Nov;13(11):965-72. Epub 2006 Oct 22. PMID:17057717
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