2mrb
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(New page: 200px<br /><applet load="2mrb" size="450" color="white" frame="true" align="right" spinBox="true" caption="2mrb" /> '''THREE-DIMENSIONAL STRUCTURE OF RABBIT LIVER ...)
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Revision as of 10:38, 21 November 2007
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THREE-DIMENSIONAL STRUCTURE OF RABBIT LIVER CD-7 METALLOTHIONEIN-2A IN AQUEOUS SOLUTION DETERMINED BY NUCLEAR MAGNETIC RESONANCE
Overview
In previous work the metal-polypeptide co-ordinative bonds in the major, protein species of a reconstituted [113Cd7]metallothionein-2 preparation, from rabbit liver in aqueous solution were determined, the secondary, polypeptide structure was found to contain several half-turns and, 3(10)-helical segments, and a preliminary characterization of the overall, polypeptide backbone fold in the beta-domain containing the three-metal, cluster, and the alpha-domain containing the four-metal cluster, was, obtained. Using a new, more extensive set of nuclear magnetic resonance, data these earlier structures were improved by new structure calculations., The new experimental data consist of distance constraints from, measurements of nuclear Overhauser effects, and dihedral angle constraints, derived from both coupling constants and nuclear Overhauser effects. The, structure calculations were performed with the program DISMAN. Since no, information on the orientation of the two domains relative to each other, could be obtained, the structure calculations were performed separately, for the alpha-domain and the beta-domain. The average of the pairwise, root-mean-square distances among the 20 structures with the least residual, violations of input constraints was 2.9 A for the beta-domain and 1.4 A, for the alpha-domain (1 A = 0.1 nm). The overall chirality of the, polypeptide fold is right-handed for the beta-domain and left-handed for, the alpha-domain. For each of the seven metal ions the local chirality of, the co-ordination of the four cysteinyl Sy atoms is clearly defined. The, improved structures of both domains show the previously noted differences, relative to the recently published crystal structure of metallothionein-2a, from rat liver.
About this Structure
2MRB is a Single protein structure of sequence from Oryctolagus cuniculus with CD as ligand. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of rabbit liver [Cd7]metallothionein-2a in aqueous solution determined by nuclear magnetic resonance., Arseniev A, Schultze P, Worgotter E, Braun W, Wagner G, Vasak M, Kagi JH, Wuthrich K, J Mol Biol. 1988 Jun 5;201(3):637-57. PMID:3418714
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