2nmb
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(New page: 200px<br /><applet load="2nmb" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nmb" /> '''DNUMB PTB DOMAIN COMPLEXED WITH A PHOSPHOTYR...)
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Revision as of 10:40, 21 November 2007
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DNUMB PTB DOMAIN COMPLEXED WITH A PHOSPHOTYROSINE PEPTIDE, NMR, ENSEMBLE OF STRUCTURES.
Overview
The phosphotyrosine-binding (PTB) domain of Numb, a protein involved in, asymmetric cell division, has recently been shown to bind to the adapter, protein Lnx through an LDNPAY sequence, to the Numb-associated kinase, (Nak) through a sequence that does not contain an NPXY motif and to, GP(p)Y-containing peptides obtained from library screening. We show here, that these diverse peptide sequences bind with comparable affinities to, the Numb PTB domain at a common binding site on the surface of the, protein. The NMR structure of the Numb PTB domain in complex with a, GPpY-containing peptide reveals a novel mechanism of binding with the, peptide in a helical turn that does not hydrogen bond to the PTB domain, beta-sheet. These results suggest that PTB domains can potentially have, multiple modes of peptide recognition and provide a structural basis from, which the multiple functions of the Numb PTB domain during asymmetric cell, division could arise.
About this Structure
2NMB is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Structure of a Numb PTB domain-peptide complex suggests a basis for diverse binding specificity., Li SC, Zwahlen C, Vincent SJ, McGlade CJ, Kay LE, Pawson T, Forman-Kay JD, Nat Struct Biol. 1998 Dec;5(12):1075-83. PMID:9846878
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