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2nnw
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(New page: 200px<br /><applet load="2nnw" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nnw, resolution 2.7Å" /> '''Alternative conformat...)
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Revision as of 10:41, 21 November 2007
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Alternative conformations of Nop56/58-fibrillarin complex and implication for induced-fit assenly of box C/D RNPs
Overview
The Nop56/58-fibrillarin heterocomplex is a core protein complex of the, box C/D ribonucleoprotein particles that modify and process ribosomal, RNAs. The previous crystal structure of the Archaeoglobus fulgidus complex, revealed a symmetric dimer of two Nop56/58-fibrillarin complexes linked by, the coiled-coil domains of the Nop56/68 proteins. However, because the A., fulgidus Nop56/58 protein lacks some domains found in most other species, it was thought that the bipartite architecture of the heterocomplex was, not likely a general phenomenon. Here we report the crystal structure of, the Nop56/58-fibrillarin complex bound with methylation cofactor, S-adenosyl-L-methionine from Pyrococcus furiosus, at 2.7 A. The new, complex confirms the generality of the previously observed bipartite, arrangement. In addition however, the conformation of Nop56/58 in the new, structure differs substantially from that in the earlier structure. The, distinct conformations of Nop56/58 suggest potential flexibility in, Nop56/58. Computational normal mode analysis supports this view., Importantly, fibrillarin is repositioned within the two complexes. We, propose that hinge motion within Nop56/58 has important implications for, the possibility of simultaneously positioning two catalytic sites at the, two target sites of a bipartite box C/D guide RNA.
About this Structure
2NNW is a Protein complex structure of sequences from Pyrococcus furiosus. Full crystallographic information is available from OCA.
Reference
Alternative Conformations of the Archaeal Nop56/58-Fibrillarin Complex Imply Flexibility in Box C/D RNPs., Oruganti S, Zhang Y, Li H, Robinson H, Terns MP, Terns RM, Yang W, Li H, J Mol Biol. 2007 Aug 31;371(5):1141-50. Epub 2007 Jun 15. PMID:17617422
Page seeded by OCA on Wed Nov 21 12:48:42 2007
