2ns1

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Revision as of 10:45, 21 November 2007

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spinqualitylabelsall models 2ns1, resolution 1.962Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of the e. coli ammonia channel AMTB complexed with the signal transduction protein GLNK

Overview

Ammonia conductance is highly regulated. A P(II) signal transduction, protein, GlnK, is the final regulator of transmembrane ammonia conductance, by the ammonia channel AmtB in Escherichia coli. The complex formed, between AmtB and inhibitory GlnK at 1.96-A resolution shows that the, trimeric channel is blocked directly by GlnK and how, in response to, intracellular nitrogen status, the ability of GlnK to block the channel is, regulated by uridylylation/deuridylylation at Y51. ATP and Mg(2+) augment, the interaction of GlnK. The hydrolyzed product, adenosine 5'-diphosphate, orients the surface of GlnK for AmtB blockade. 2-Oxoglutarate diminishes, AmtB/GlnK association, and sites for 2-oxoglutarate are evaluated.

About this Structure

2NS1 is a Protein complex structure of sequences from Escherichia coli with BOG, ADP and TRS as ligands. Full crystallographic information is available from OCA.

Reference

Inhibitory complex of the transmembrane ammonia channel, AmtB, and the cytosolic regulatory protein, GlnK, at 1.96 A., Gruswitz F, O'Connell J 3rd, Stroud RM, Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):42-7. Epub 2006 Dec 26. PMID:17190799

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