2o1x
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(New page: 200px<br /><applet load="2o1x" size="450" color="white" frame="true" align="right" spinBox="true" caption="2o1x, resolution 2.900Å" /> '''1-deoxy-D-xylulose ...)
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Revision as of 10:54, 21 November 2007
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1-deoxy-D-xylulose 5-phosphate synthase (DXS) from Deinococcus radiodurans
Overview
Isopentenyl pyrophosphate (IPP) is a common precursor for the synthesis of, all isoprenoids, which have important functions in living organisms. IPP, is produced by the mevalonate pathway in archaea, fungi, and animals. In, contrast, IPP is synthesized by a mevalonate-independent pathway in most, bacteria, algae, and plant plastids. 1-Deoxy-D-xylulose 5-phosphate, synthase (DXS) catalyzes the first and the rate-limiting step of the, mevalonate-independent pathway and is an attractive target for the, development of novel antibiotics, antimalarials, and herbicides. We report, here the first structural information on DXS, from Escherichia coli and, Deinococcus radiodurans, in complex with the coenzyme thiamine, pyrophosphate (TPP). The structure contains three domains (I, II, and, III), each of which bears homology to the equivalent domains in, transketolase and the E1 subunit of pyruvate dehydrogenase. However, DXS, has a novel arrangement of these domains as compared with the other, enzymes, such that the active site of DXS is located at the interface of, domains I and II in the same monomer, whereas that of transketolase is, located at the interface of the dimer. The coenzyme TPP is mostly buried, in the complex, but the C-2 atom of its thiazolium ring is exposed to a, pocket that is the substrate-binding site. The structures identify, residues that may have important roles in catalysis, which have been, confirmed by our mutagenesis studies.
About this Structure
2O1X is a Single protein structure of sequence from Deinococcus radiodurans with MG and TDP as ligands. Active as 1-deoxy-D-xylulose-5-phosphate synthase, with EC number 2.2.1.7 Full crystallographic information is available from OCA.
Reference
Crystal structure of 1-deoxy-D-xylulose 5-phosphate synthase, a crucial enzyme for isoprenoids biosynthesis., Xiang S, Usunow G, Lange G, Busch M, Tong L, J Biol Chem. 2007 Jan 26;282(4):2676-82. Epub 2006 Nov 29. PMID:17135236
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