2ob2
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(New page: 200px<br /><applet load="2ob2" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ob2, resolution 1.920Å" /> '''ppm1 in the absence...)
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Revision as of 11:00, 21 November 2007
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ppm1 in the absence of 1,8-ANS (cf 1JD)
Overview
A technique is described whereby the addition of low concentrations, (millimolar to micromolar) of the fluorescent dye 1,8-ANS to the protein, solution prior to crystallization results in crystallization experiments, in which protein crystals are strongly contrasted above background, artifacts when exposed to low-intensity UV radiation. As 1,8-ANS does not, covalently modify the protein sample, no further handling or purification, steps are necessary. The system has been tested on a wide variety of, protein samples and it has been shown that the addition of 1,8-ANS has no, discernible effect on the crystallization frequencies or crystallization, conditions of these proteins. As 1,8-ANS interacts with a wide variety of, proteins, this is proposed to be a general solution for the automated, classification of protein crystallization images and the detection of, protein crystals. The results also demonstrate the expected discrimination, between salt and protein crystals, as well as allowing the straightforward, identification of small crystals that grow in precipitate or under a, protein skin.
About this Structure
2OB2 is a Single protein structure of sequence from Saccharomyces cerevisiae with PO4, SAH and GOL as ligands. Full crystallographic information is available from OCA.
Reference
A method for the general identification of protein crystals in crystallization experiments using a noncovalent fluorescent dye., Groves MR, Muller IB, Kreplin X, Muller-Dieckmann J, Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):526-35. Epub 2007, Mar 16. PMID:17372358
Page seeded by OCA on Wed Nov 21 13:07:18 2007
