This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2oqn
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="2oqn" size="450" color="white" frame="true" align="right" spinBox="true" caption="2oqn, resolution 1.90Å" /> '''High Pressure Cryoco...)
Next diff →
Revision as of 11:08, 21 November 2007
|
High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths
Overview
Crystal cryocooling is usually employed to reduce radiation damage during, X-ray crystallography. Recently, a high-pressure cryocooling method has, been developed which results in excellent diffraction-quality crystals, without the use of penetrative cryoprotectants. Three new developments of, the method are presented here: (i) Xe-He high-pressure cryocooling for Xe, SAD phasing, (ii) native sulfur SAD phasing and (iii) successful, cryopreservation of crystals in thick-walled capillaries without, additional cryoprotectants other than the native mother liquor. These, developments may be useful for structural solution of proteins without the, need for selenomethionine incorporation and for high-throughput protein, crystallography.
About this Structure
2OQN is a Single protein structure of sequence from Thaumatococcus daniellii with TAR as ligand. Full crystallographic information is available from OCA.
Reference
High-pressure cryocooling for capillary sample cryoprotection and diffraction phasing at long wavelengths., Kim CU, Hao Q, Gruner SM, Acta Crystallogr D Biol Crystallogr. 2007 May;63(Pt 5):653-9. Epub 2007, Apr 21. PMID:17452791
Page seeded by OCA on Wed Nov 21 13:15:27 2007
