NS5B

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	[[Image:PolyProtein.jpg]]		[[Image:PolyProtein.jpg]]
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		+	== '''NS5B + LIGANDS''' ==
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		+	<applet load='2HAI_ligands.pdb' size='300' frame='true' align='right' caption='NS5B' />

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Revision as of 07:18, 20 April 2009

Contents 1 RNA Dependent RNA Polymerase from Hepatitis C Virus 2 NS5B with active site carboxylates highlighted 3 NS5B with peptide segments known to bind RNA highlighted 4 NS5B + LIGANDS

RNA Dependent RNA Polymerase from Hepatitis C Virus

spinqualitylabelsall models PDB ID 2HAI Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Placeholder, type here.

Image:2HAI domains.jpg

NS5B with active site carboxylates highlighted

spinqualitylabelsall models PDB IDs 2HAI (protein) 1RTD (DNA) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

NS5B with B form DNA from HIV Reverse Transcriptase co-crystal model. DNA was modeled in by alignment of palms domain. A beta hairpin in the thumb domain has been moved to accommodate DNA, the hairpin is modeled into the minor groove, a possible binding site. Catalytic Mg2+ ions are modeled in green, these would be coordinated by the three aspartic acid carboxylates, (D220, D318 and D319).

The template strand is seen entering through a gap in the fingers domain, incoming nucleotide that extends the primer lines up with the NS5B active site and duplex DNA exits the enzyme through the large central hole.

Image:2HAI RNA binders.jpg

NS5B with peptide segments known to bind RNA highlighted

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Image:PolyProtein.jpg

NS5B + LIGANDS

spinqualitylabelsall models NS5B Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image