NS5B

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<applet load='2HAI_catalytic3.pdb' size='600' frame='true' align='right' caption='PDB ID 2HAI' />Placeholder, type here.
<applet load='2HAI_catalytic3.pdb' size='600' frame='true' align='right' caption='PDB ID 2HAI' />Placeholder, type here.
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<scene name='NS5B/Native_ns5b/2'>TextToBeDisplayed</scene>
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<scene name='NS5B/Native_ns5b/2'>3 active site aspartates</scene>
[[Image:2HAI_domains.jpg]]
[[Image:2HAI_domains.jpg]]

Revision as of 18:53, 20 April 2009

Contents

RNA Dependent RNA Polymerase from Hepatitis C Virus

PDB ID 2HAI

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Placeholder, type here.

Image:2HAI domains.jpg

NS5B with active site carboxylates highlighted

PDB IDs 2HAI (protein) 1RTD (DNA)

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NS5B with B form DNA from HIV Reverse Transcriptase co-crystal model. DNA was modeled in by alignment of palms domain. A beta hairpin in the thumb domain has been moved to accommodate DNA, the hairpin is modeled into the minor groove, a possible binding site. Catalytic Mg2+ ions are modeled in green, these would be coordinated by the three aspartic acid carboxylates, (D220, D318 and D319).

The template strand is seen entering through a gap in the fingers domain, incoming nucleotide that extends the primer lines up with the NS5B active site and duplex DNA exits the enzyme through the large central hole.

Image:2HAI RNA binders.jpg

NS5B with peptide segments known to bind RNA highlighted

Image:PolyProtein.jpg

NS5B + LIGANDS

NS5B

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