User:Karl E. Zahn/Sandbox RB69 1/fingers-domain
From Proteopedia
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| - | The fingers domain of RB69 DNA pol exist as an insertion between the two sequences forming the palm. Since the fingers domain experiences a 60 degree rotation from the open or apo structure to the closed catalytically competent structure, the fact that the palm flanks the fingers is likely required for proper communication between these domains. The fingers domain also provides important basic residues that bind triphosphates. K561 is also believed to stabilize the pentacovalent intermediate formed with nucleophilic attack of the 3'-OH on the alpha phosphate. | ||
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[[Image:1IG9 1WAJ fingers.png]] | [[Image:1IG9 1WAJ fingers.png]] | ||
| + | The fingers domain of RB69 DNA pol exist as an insertion between the two sequences forming the palm. Since the fingers domain experiences a 60 degree rotation from the open or apo structure to the closed catalytically competent structure, the fact that the palm flanks the fingers in primary sequence is likely required for proper communication between these domains. The fingers domain also provides important <scene name='User:Karl_E._Zahn/Sandbox_RB69_1/fingers-domain/Nucleotide-binding/1'>basic residues</scene> that bind triphosphates. K561 is also believed to stabilize the pentacovalent intermediate formed with nucleophilic attack of the 3'-OH on the alpha phosphate<ref>PMID:11389835</ref>. | ||
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| + | <applet load='Insert PDB code or filename here' size='300' frame='true' align='right' caption='Insert caption here scene='User:Karl_E._Zahn/Sandbox_RB69_1/fingers-domain/Fingers/1' /> | ||
Revision as of 13:32, 23 April 2009
The fingers domain of RB69 DNA pol exist as an insertion between the two sequences forming the palm. Since the fingers domain experiences a 60 degree rotation from the open or apo structure to the closed catalytically competent structure, the fact that the palm flanks the fingers in primary sequence is likely required for proper communication between these domains. The fingers domain also provides important that bind triphosphates. K561 is also believed to stabilize the pentacovalent intermediate formed with nucleophilic attack of the 3'-OH on the alpha phosphate[1].
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