User:Susana Retamal/Sandbox1
From Proteopedia
(Difference between revisions)
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=Background Information= | =Background Information= | ||
| - | Rsp1275 is a member of the Fnr-Crp family of transcriptional regulators. Homologues of the Fnr protein from ''E. coli'' have been identified in a variety of taxonomically <1> diverse bacterial species. The bacterium ''Rhodobacter spheroides 2.4.1'' encodes 8 members of this family, however only 2 have known function. The modeling of 7 of them were successfully obtained using 3D-Jigsaw, all being very similar in structure to Crp (the only one with crystal structure solved). The DNA binding domain helix-turn-helix is located in the C-terminal, and the N-terminal part contains the allosteric effector domain. Rsp1275 has been linked with salt-tolerance in ''Rhodobacter spheroides''. Different genes coding for the Fnr-Crp proteins of unknown function have been cloned and a multicopy test was performed to evaluate these as potential regulators of NaCl-responsive genes. | + | Rsp1275 is a member of the Fnr-Crp family of transcriptional regulators. Homologues of the Fnr protein from ''E. coli'' have been identified in a variety of taxonomically <ref>1) Spiro, S. 1994. <u>The FNR family of transcriptional regulators.</u>. Antonie van Leeuwenhoek 66:23–36. <u> diverse bacterial species. The bacterium ''Rhodobacter spheroides 2.4.1'' encodes 8 members of this family, however only 2 have known function. The modeling of 7 of them were successfully obtained using 3D-Jigsaw, all being very similar in structure to Crp (the only one with crystal structure solved). The DNA binding domain helix-turn-helix is located in the C-terminal, and the N-terminal part contains the allosteric effector domain. Rsp1275 has been linked with salt-tolerance in ''Rhodobacter spheroides''. Different genes coding for the Fnr-Crp proteins of unknown function have been cloned and a multicopy test was performed to evaluate these as potential regulators of NaCl-responsive genes. |
''Structure of the Proposed rsp1275'''<applet load='rsp1275.pdb' size='300' color='black' frame='true' align='right' caption='3D Image of proposed rsp1275 protein'/> | ''Structure of the Proposed rsp1275'''<applet load='rsp1275.pdb' size='300' color='black' frame='true' align='right' caption='3D Image of proposed rsp1275 protein'/> | ||
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Theoretical pI: 6.10 | Theoretical pI: 6.10 | ||
| - | Globe Prediction: it appears as compact, as a globular doimain. | + | Globe Prediction: it appears as compact, as a globular doimain. <ref>2)PredictProtein: B Rost, G Yachdav and J Liu (2004) <u> |
Secondary Structure: | Secondary Structure: | ||
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| - | =References= | + | ==References== |
| - | + | 1) Spiro, S. 1994. <u>The FNR family of transcriptional regulators.</u>. Antonie van Leeuwenhoek 66:23–36. | |
| - | + | 2) PredictProtein: B Rost, G Yachdav and J Liu (2004) <u> The PredictProtein Server.</u> Nucleic Acids Research 32(Web Server issue):W321-W326. | |
Revision as of 14:24, 27 April 2009
- User:Susana Retamal/Sandbox1
Background Information
Rsp1275 is a member of the Fnr-Crp family of transcriptional regulators. Homologues of the Fnr protein from E. coli have been identified in a variety of taxonomically [1]
